Cytokeratin 20 Antibody Protein Microarray Validated / KRT20 Antibody [clone KRT20/1992] (V3909)

Cytokeratin 20 Antibody for IHC. Immunohistochemistry analysis of human colon tissue using protein microarray validated Cytokeratin 20 antibody (clone KRT20/1992). HRP-DAB brown chromogenic staining highlights cytoplasmic Keratin 20 / Cytokeratin 20 (KRT20) expression in epithelial cells lining colonic glandular crypts, while surrounding stromal cells remain largely negative. This staining pattern reflects the expected epithelial localization of this intermediate filament protein in gastrointestinal mucosa. Required HIER: boil tissue sections in 10 mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of human colon carcinoma with Cytokeratin 20 antibody (clone KRT20/1992). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human HT29 cell lysate with Cytokeratin 20 antibody (clone KRT20/1992). Predicted molecular weight ~46 kDa.

Cytokeratin 20 Antibody Protein Microarray Validated. HuProt(TM) human protein microarray specificity analysis using Cytokeratin 20 Antibody Protein Microarray Validated clone KRT20/1992. The microarray contains more than 19,000 full-length human proteins. KRT20 shows the strongest signal with the highest Z-score and S-score, indicating highly selective recognition of Keratin 20 / KRT20 relative to other proteins on the array. Z-score represents the signal intensity generated when the antibody binds to a specific protein spot, expressed as standard deviations above the mean signal of the array. The S-score represents the difference between adjacent Z-scores and reflects the relative specificity of the antibody for its intended target protein.