Cytokeratin 16 Antibody LL025 / CK16 Monoclonal Antibody [clone LL025] (V7921)

Cytokeratin 16 Antibody LL025 by IHC in human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded human tonsil tissue using Cytokeratin 16 Antibody clone LL025. Brown immunoreactivity is concentrated within epithelial structures, highlighting the cytoplasm of cells associated with the stratified squamous epithelial compartment. The surrounding lymphoid tissue exhibits comparatively little staining, producing a clear distinction between epithelial and nonepithelial regions. Hematoxylin counterstaining reveals nuclei in blue. The CK16 Monoclonal Antibody was applied following heat-induced epitope retrieval by boiling tissue sections in 10 mM Tris containing 1 mM EDTA, pH 9, for 20 minutes, followed by cooling before staining.

Cytokeratin 16 Antibody LL025 purity by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free Cytokeratin 16 Antibody clone LL025 under reduced (R) and non-reduced (NR) conditions. The reduced sample displays prominent bands migrating at approximately 50 kDa and 25 kDa, corresponding to immunoglobulin heavy and light chains. The non-reduced sample exhibits a prominent band near 150 kDa, consistent with intact immunoglobulin. Molecular weight standards are included for reference. This analysis evaluates the integrity and purity of the CK16 Monoclonal Antibody preparation rather than detection of endogenous cytokeratin 16 protein.