KRT15 Antibody KRT15/2554 / Cytokeratin 15 Monoclonal Antibody [clone KRT15/2554] (V7428)

KRT15 Antibody KRT15/2554 by IHC in human basal cell carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human basal cell carcinoma tissue using the Cytokeratin 15 Monoclonal Antibody. Strong brown immunoreactivity is visible within multiple nests of basaloid tumor cells, with staining concentrated in the epithelial tumor structures. The surrounding stromal tissue exhibits comparatively limited labeling, while hematoxylin counterstaining highlights nuclei in blue. The staining distribution demonstrates KRT15 immunoreactivity within the examined neoplastic epithelial population. Heat-induced epitope retrieval was performed using 10 mM citrate buffer, pH 6, for 10-20 minutes.

KRT15 Antibody KRT15/2554 by IHC in human basal cell carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human basal cell carcinoma tissue using the Cytokeratin 15 Monoclonal Antibody. Prominent brown staining follows a continuous, irregular band of epithelial tumor cells along the tissue interface, with comparatively little immunoreactivity in the adjacent connective tissue. The sharply demarcated staining pattern highlights KRT15 expression within the basaloid epithelial compartment, while hematoxylin counterstaining identifies nuclei in blue. Heat-induced epitope retrieval was performed using 10 mM citrate buffer, pH 6, for 10-20 minutes.

KRT15 Antibody KRT15/2554 by IHC in human prostate carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human prostate carcinoma tissue using the Cytokeratin 15 Monoclonal Antibody. Brown immunoreactivity is concentrated along the epithelial lining of several irregular glandular structures, with staining distributed primarily within the cytoplasm of the epithelial cells. The surrounding stromal regions show comparatively weak labeling, while hematoxylin counterstaining highlights nuclei in blue. The staining pattern demonstrates KRT15 immunoreactivity in selected epithelial structures within the examined tissue. Heat-induced epitope retrieval was performed using 10 mM citrate buffer, pH 6, for 10-20 minutes.

KRT15 Antibody KRT15/2554 by FACS in human HeLa cells. Flow cytometry analysis of fixed and permeabilized HeLa human cervical carcinoma cells using the Cytokeratin 15 Monoclonal Antibody. The blue histogram represents antibody staining, while the red histogram represents the isotype control. A clear rightward fluorescence shift is observed in the antibody-stained population, with the blue distribution centered at a higher fluorescence intensity than the control. The separation between the two populations demonstrates increased antibody-associated fluorescence under the experimental conditions, supporting detection of intracellular KRT15 in the examined HeLa cell population.

KRT15 Antibody KRT15/2554 by protein microarray analysis. Protein microarray specificity assessment of the Cytokeratin 15 Monoclonal Antibody using a HuProt array containing more than 19,000 full-length human proteins. KRT15 produces the highest-ranking signal, with a Z-score of approximately 67.4, followed by substantially weaker signals from other proteins, including ZFP423 and TAF10. The pronounced separation between the intended target and lower-ranked interactions supports preferential recognition of KRT15 under the assay conditions. Z-scores represent signal strength relative to the array mean, while S-scores describe the separation between ranked signals. These findings support the designation Protein Microarray Validated.

SDS-PAGE analysis of purified, BSA-free Cytokeratin 15 antibody (clone KRT15/2554) as confirmation of integrity and purity.