Cytokeratin 1 Antibody LHK1 / CK1 Monoclonal Antibody [clone LHK1] (V7907)

Cytokeratin 1 Antibody LHK1 by IHC in Human Cervix. Immunohistochemistry of FFPE human cervical tissue using Cytokeratin 1 Antibody LHK1. Moderate to strong brown DAB staining is observed predominantly in the cytoplasm of differentiated squamous epithelial cells, with stronger expression in the superficial epithelial layers and relatively weak staining in the basal region. The CK1 Monoclonal Antibody demonstrates the differentiation-associated expression pattern of KRT1 in stratified squamous epithelium. Nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 20 minutes, followed by cooling before antibody incubation.

Cytokeratin 1 Antibody LHK1 by IHC in Human Skin. Immunohistochemistry of FFPE human skin tissue using Cytokeratin 1 Antibody LHK1. Strong brown DAB staining is observed throughout the suprabasal epidermal layers, including the spinous and granular regions, while the basal keratinocyte layer shows comparatively little staining. The CK1 Monoclonal Antibody highlights the characteristic distribution of KRT1 in differentiating keratinocytes, consistent with its role in epidermal intermediate filament organization. Nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 20 minutes, followed by cooling before antibody incubation.

Cytokeratin 1 Antibody LHK1 by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free Cytokeratin 1 Antibody LHK1 under non-reducing (NR) and reducing (R) conditions, with 2 ug of antibody loaded per lane. The non-reducing lane shows a prominent band migrating at approximately 150 kDa, consistent with intact IgG. Under reducing conditions, bands corresponding to the immunoglobulin heavy chain (~50 kDa) and light chain (~25 kDa) are visible. The electrophoretic profile is consistent with the expected composition of a purified mouse monoclonal antibody.