• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> CYP27B1 Antibody

CYP27B1 Antibody (F54519)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image F54519-0.2ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.2 ml 429
Image
F54519-0.05ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.05 ml 199
Bulk quote request
IHC testing of FFPE human kidney tissue with CYP27B1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
›
IHC testing of FFPE human kidney tissue with CYP27B1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human T-47D cell lysate with CYP27B1 antibody. Predicted molecular weight ~57 kDa.
Western blot testing of rat kidney lysate with CYP27B1 antibody. Predicted molecular weight ~57 kDa.
Flow cytometry testing of fixed and permeabilized human HeLa cells with CYP27B1 antibody; Blue=isotype control, Green= CYP27B1 antibody.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt O15528
Localization Cytoplasmic
Applications Western Blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:25
Flow Cytometry : 1:25 (1x10e6 cells)
Limitations This CYP27B1 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

  • Applications : WB, IHC-P
    Reactivity : Human, Mouse, Rat
  • Applications : WB, Direct ELISA
    Reactivity : Human

Description

CYP27B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.

Application Notes

The stated application concentrations are suggested starting points. Titration of the CYP27B1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 482-508 from the human protein was used as the immunogen for the CYP27B1 antibody.

Storage

Aliquot the CYP27B1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Related Products

   1.   View all CYP27B1 antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.