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Home >> Antibodies >> CYP24A1 Antibody

CYP24A1 Antibody (R32199)

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  Catalog No Formulation Size Price (USD)  
Image R32199 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 419
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Western blot testing of 1) rat thymus, 2) rat lung, 3) HeLa, 4) 22RV1, 5) A431 and 6) mouse HEPA lysate with CYP24A1 antibody. Predicted/observed molecular weight ~59 kDa.
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Western blot testing of 1) rat thymus, 2) rat lung, 3) HeLa, 4) 22RV1, 5) A431 and 6) mouse HEPA lysate with CYP24A1 antibody. Predicted/observed molecular weight ~59 kDa.
IHC testing of FFPE human pancreatic cancer tissue with CYP24A1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE mouse kidney with CYP24A1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat kidney with CYP24A1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt Q07973
Localization Cytoplasmic
Applications Western blot : 0.1-0.5ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This CYP24A1 antibody is available for research use only.
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  • Applications : WB, ELISA (peptide)
    Reactivity : Human, Rat

Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. And this mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Application Notes

Optimal dilution of the CYP24A1 antibody should be determined by the researcher.

Immunogen

Amino acids 153-514 of human CYP24A1 were used as the immunogen for the CYP24A1 antibody.

Storage

After reconstitution, the CYP24A1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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