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Home >> Antibodies >> Cyclin D1 Antibody / PE Conjugate

Cyclin D1 Antibody / PE Conjugate [clone BCLD1-1] (V7043PE)

  Catalog No Formulation Size Price (USD)  
Image V7043PE-100T 500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 Tests 499
FACS testing of human HeLa cells: Black=cells alone; Green=isotype control; Red=Cyclin D1 antibody
Availability 1-3 business days
Species Reactivity Human
Format PE Conjugate
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name BCLD1-1
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 595
Localization Nuclear
Applications Flow Cytometry : 5ul/test/million cells or 5ul/test/100ul of whole blood
Limitations This Cyclin D1 antibody is available for research use only.
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Description

This antibody recognizes a protein of 36kDa, identified as Cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression. The antibody is useful in identifying mantle cell lymphomas (Cyclin D1 positive) from CLL/SLL and follicular lymphomas (Cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Application Notes

The dilution stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cyclin D1 antibody to be titered up or down for optimal performance.

Immunogen

Human recombinant full length Cyclin D1 protein was used as the immunogen.

Storage

Store the Cyclin D1 antibody at 2-8oC. Conjugate is light sensitive, store in the dark.

Alternate Names

Bcl-1, Bcl1

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