Collagen I Antibody / COL1A1 [clone AFD-3] (RQ5202)

Collagen I Antibody Human Lung Squamous Cell Carcinoma IHC. IHC staining of FFPE human lung squamous cell carcinoma tissue with Collagen I antibody demonstrated strong extracellular staining within the tumor-associated stroma and desmoplastic connective tissue surrounding malignant cell nests. Collagen I is the principal fibrillar collagen of the extracellular matrix and provides structural support while influencing cell adhesion, migration, and tissue remodeling. The prominent stromal staining observed is consistent with the extensive extracellular matrix deposition and remodeling commonly associated with squamous cell carcinoma progression. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 10-20 minutes and allowing them to cool before testing. These results support the utility of Collagen I Antibody for studies of extracellular matrix biology, tumor stroma formation, fibrosis, and cancer-associated tissue remodeling.

Collagen I Antibody Human Colorectal Adenocarcinoma IHC. IHC staining of FFPE human colorectal adenocarcinoma tissue with Collagen I antibody demonstrated strong extracellular staining within the tumor-associated stroma and fibrotic connective tissue surrounding malignant glandular structures. Collagen I is the major fibrillar collagen of the extracellular matrix and plays critical roles in tissue architecture, mechanical support, and cell-matrix interactions. The prominent stromal staining observed is consistent with desmoplastic extracellular matrix remodeling frequently associated with colorectal adenocarcinoma progression and tumor microenvironment formation. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 10-20 minutes and allowing them to cool before testing. These results support the utility of Collagen I Antibody for studies of extracellular matrix biology, tumor stroma development, fibrosis, and cancer-associated tissue remodeling.

IHC staining of FFPE human placental tissue with Collagen I antibody. HIER: boil tissue sections in pH8 EDTA buffer, for 10-20 min and allow to cool before testing.

IHC staining of FFPE human breast cancer tissue with Collagen I antibody. HIER: boil tissue sections in pH8 EDTA buffer, for 10-20 min and allow to cool before testing.

Western blot testing of human stomach lysate with Collagen I antibody. Expected molecular weight: 100-140 kDa.