CK15 Antibody KRT15/2959 / Cytokeratin 15 Monoclonal Antibody [clone KRT15/2959] (V7919)

CK15 Antibody KRT15/2959 by IHC in human prostate carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human prostate carcinoma tissue using the Cytokeratin 15 Monoclonal Antibody. Brown immunoreactivity is concentrated within epithelial structures, with prominent staining along the cytoplasm of cells lining glandular spaces. Surrounding stromal regions exhibit comparatively limited labeling, while hematoxylin counterstaining highlights nuclei in blue. The distribution demonstrates CK15 immunoreactivity within epithelial compartments of the examined tissue. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 minutes.

IHC staining of FFPE human prostate carcinoma with CK15 antibody (clone KRT15/2959). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

CK15 Antibody KRT15/2959 by IF in human MCF7 cells. Immunofluorescence staining of methanol-fixed MCF7 human breast cancer cells using the Cytokeratin 15 Monoclonal Antibody. Cyan-green fluorescence is distributed throughout the cytoplasm, forming a filamentous network surrounding the nuclei and extending toward the cell periphery. RedDot nuclear counterstaining appears red, providing contrast with the cytoplasmic antibody signal. The observed filamentous staining pattern is consistent with the organization of keratin intermediate filaments in epithelial cells. Individual cells display varying fluorescence intensity, with prominent cytoplasmic networks visible throughout the examined cell population.

CK15 Antibody KRT15/2959 by WB in human thymus tissue. Western blot analysis of human thymus tissue lysate using the Cytokeratin 15 Monoclonal Antibody. A single prominent immunoreactive band is detected at approximately 50 kDa, consistent with the predicted molecular weight of human Cytokeratin 15. The band is sharply defined, with minimal background signal and no additional prominent bands visible across the molecular weight range shown. KRT15 is a type I intermediate filament protein associated with basal epithelial cells and specialized follicular compartments. The observed migration supports detection of a protein corresponding to the expected molecular weight of human CK15 under the experimental conditions.

CK15 Antibody KRT15/2959 by protein microarray analysis. Protein microarray specificity assessment of the Cytokeratin 15 Monoclonal Antibody using a HuProt array containing more than 19,000 full-length human proteins. The graph identifies KRT15 as the highest-ranking target, with a Z-score of approximately 38.9 and substantial separation from lower-ranked interactions. This preferential signal supports recognition of the intended target under the assay conditions. Z-scores indicate fluorescence signal strength relative to the array mean, while S-scores describe the separation between ranked signals. These findings support the designation Protein Microarray Validated.

SDS-PAGE analysis of purified, BSA-free CK15 antibody (clone KRT15/2959) as confirmation of integrity and purity.