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Home >> Antibodies >> CDR2 Antibody

CDR2 Antibody (RQ5966)

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  Catalog No Formulation Size Price (USD)  
Image RQ5966 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human breast cancer with CDR2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human breast cancer with CDR2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with CDR2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with CDR2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) Caco-2, 3) HEK293 and 4) A549 lysate with CDR2 antibody. Expected molecular weight: 52-62 kDa.
Flow cytometry testing of human HepG2 cells with CDR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDR2 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q01850
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry : 1-2ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This CDR2 antibody is available for research use only.
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    Reactivity : Human, Mouse

Description

Cerebellar degeneration-related protein 2 is a protein that in humans is encoded by the CDR2 gene. By a combination of study of rodent/human somatic cell hybrids and in situ hybridization, this gene is assigned to 16p13.1-p12. The onconeuronal cerebellar degeneration-related antigen Cdr2 is associated with paraneoplastic syndromes. Neoplastic expression of Cdr2 in ovary and breast tumors triggers an autoimmune response that suppresses tumor growth by developing tumor immunity, but culminates in cerebellar degeneration when Cdr2-specific immune cells recognize neuronal Cdr2.

Application Notes

Optimal dilution of the CDR2 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids M1-D441) was used as the immunogen for the CDR2 antibody.

Storage

After reconstitution, the CDR2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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