CDK5 Antibody / Cyclin Dependent Kinase 5 Antibody [clone 1552CT262.105.8] (F54486)

CDK5 Antibody Human Skeletal Muscle IHC. Immunohistochemical staining of FFPE human skeletal muscle tissue with CDK5 antibody. Heat-induced epitope retrieval (HIER) was performed by steaming tissue sections in pH 6 citrate buffer for 20 minutes prior to staining. CDK5 immunoreactivity is observed predominantly within the cytoplasm of skeletal muscle fibers, consistent with the expected expression of Cyclin Dependent Kinase 5 (CDK5), a serine/threonine protein kinase that regulates protein phosphorylation, cytoskeletal organization, intracellular signaling, and neuromuscular function. Cell nuclei are counterstained with hematoxylin (blue).

CDK5 Antibody A549 Cell IF. Immunofluorescent staining of fixed and permeabilized human A549 cells with CDK5 antibody (green), anti-Actin antibody (red), and DAPI nuclear stain (blue). CDK5 immunoreactivity is observed predominantly within the cytoplasm with additional nuclear staining, consistent with the expected localization of Cyclin Dependent Kinase 5 (CDK5), a serine/threonine protein kinase that regulates protein phosphorylation, cytoskeletal organization, intracellular signaling, and neuronal development. Actin (red) highlights the cytoskeletal network and cellular morphology.

CDK5 Antibody Human and Mouse Cell WB. Western blot testing of 1) human A549, 2) human K562, 3) human HeLa, and 4) mouse NIH 3T3 cell lysates with CDK5 antibody. A distinct immunoreactive band is detected at approximately 33 kDa in all samples, consistent with the predicted molecular weight of Cyclin Dependent Kinase 5 (CDK5). The results demonstrate cross-species detection of this serine/threonine protein kinase, which regulates protein phosphorylation, cytoskeletal organization, intracellular signaling, neuronal development, and synaptic plasticity.

CDK5 Antibody K562 Cell FACS. Flow cytometric analysis of fixed and permeabilized human K562 cells stained with CDK5 antibody. Blue: isotype control. Green: CDK5 antibody. The pronounced rightward shift of the antibody-stained population relative to the isotype control demonstrates specific intracellular detection of Cyclin Dependent Kinase 5 (CDK5), a serine/threonine protein kinase that regulates protein phosphorylation, intracellular signaling, cytoskeletal organization, and neuronal development.