- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
CD8A Antibody for IF / CD8A Immunofluorescence Antibody [clone C8/468] (V2043)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V2043-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
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V2043-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
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V2043SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 | |
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V2043IHC-7ML | Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* | 7 ml | 559 |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | C8/468 |
| Purity | Protein G affinity chromatography |
| Buffer | 1X PBS, pH 7.4 |
| UniProt | P01732 |
| Gene ID | 925 |
| Localization | Cell surface |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT Immunofluorescence : 1-2ug/ml |
| Limitations | This CD8a antibody is available for research use only. |
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CD8 alpha (CD8A) is a transmembrane glycoprotein expressed on cytotoxic T lymphocytes where it functions as a co-receptor for T cell receptor signaling through interaction with MHC class I molecules. CD8A Antibody for IF is widely used to visualize cytotoxic T cells with high spatial resolution, enabling detailed mapping of immune cell localization within tissues and cellular systems. CD8A antibody, also referred to as CD8 alpha antibody or CD8 antigen antibody, is a foundational immunofluorescence marker for identifying cytotoxic T lymphocytes and studying immune architecture.
CD8A is localized to the plasma membrane of cytotoxic T cells, where it forms heterodimers with CD8B or homodimers in specific immune subsets. In immunofluorescence applications, CD8A staining produces clear membrane-associated signal outlining individual lymphocytes, allowing precise visualization of cell morphology and spatial relationships. This enables CD8A immunofluorescence antibody detection to distinguish infiltrating T cells from epithelial, stromal, and tumor compartments in complex tissue environments.
CD8A Antibody for IF is particularly valuable in spatial biology and immuno-oncology studies, where the positioning of cytotoxic T cells relative to tumor cells, vasculature, and stromal structures provides critical biological insight. Immunofluorescence detection allows detailed analysis of immune infiltration patterns, including localization within tumor nests, invasive margins, and peritumoral regions. These spatial relationships are essential for understanding immune activation, immune exclusion, and therapeutic response mechanisms.
This mouse monoclonal antibody, clone C8/468, is provided in an unlabeled format, allowing detection through fluorophore-conjugated secondary antibodies. The unlabeled CD8A antibody format provides maximum flexibility for experimental design, enabling researchers to select fluorophores based on available imaging channels and multiplex panel requirements. This makes it especially well suited for multi-marker immunofluorescence workflows where CD8A staining is combined with markers such as CD3 antibody, CD4 antibody, or checkpoint proteins including PD-1 and PD-L1.
The use of an unlabeled CD8A immunofluorescence antibody also allows signal amplification through secondary antibody binding, which can enhance detection sensitivity in samples with low antigen abundance. This feature is particularly useful in studies where CD8-positive lymphocytes are present at low density or in heterogeneous tissue environments. Additionally, the ability to swap secondary antibodies enables adaptation across different imaging platforms, including confocal microscopy and high-content imaging systems.
CD8A immunofluorescence antibody staining using an unlabeled format supports advanced co-localization studies and multiplex IF panel development, where precise control over fluorophore selection is critical. This flexibility makes CD8A Antibody for IF an essential reagent for high-resolution immune mapping, enabling detailed investigation of cytotoxic T cell distribution, immune cell interactions, and microenvironment organization in both normal and disease contexts.
This antibody is part of a broader selection of immune cell marker antibodies designed to support studies of T cell biology, immune infiltration, and tumor immunology, including application-specific CD8A antibody reagents for IHC, FACS, WB, and IF.
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD8A Antibody for IF / CD8A Immunofluorescence Antibody to be titered up or down for optimal performance.
>1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Human recombinant protein was used as the immunogen for this CD8A Antibody for IF / CD8A Immunofluorescence Antibody.
Store the CD8a antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
CD8A IF antibody, CD8 alpha immunofluorescence antibody, CD8A fluorescent staining antibody, CD8 cytotoxic T cell IF antibody, CD8A intracellular localization antibody
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