CD6 Antibody 3F7B5 | V2039 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	V2039-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	539
Formulation
	V2039-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	249
Formulation
	V2039SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	539
Formulation
	V2039IHC-7ML	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only	7 ml	539
Formulation

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Immunofluorescent staining of human MOLT-4 cells with CD6 antibody (clone 3F7B5, green) and Reddot nuclear stain (red).

IHC staining of FFPE human tonsil tissue with CD6 antibody (clone 3F7B5). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Flow cytometry staining of human MOLT-4 cells with CD6 antibody; Red=isotype control, Blue= CD6 antibody.

SDS-PAGE analysis of purified, BSA-free CD6 antibody (clone 3F7B5) as confirmation of integrity and purity.

Species Reactivity	Human
Format	Purified
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1, kappa
Clone Name	3F7B5
Purity	Protein G affinity chromatography
Gene ID	923
Localization	Cell surface and cytoplasmic
Applications	Flow Cytometry : 1-2ug/million cells Immunofluorescence : 1-3ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations	This CD6 antibody is available for research use only.
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Description

CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular scavenger receptor cysteine-rich (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. It shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. CD6 antibody can be used to deplete T cells from bone marrow transplants to prevent graft versus host disease.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be tittered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.