NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	V2039-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	459
Formulation
	V2039-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	209
Formulation
	V2039SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	459
Formulation
	V2039IHC-7ML	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only	7 ml	459
Formulation

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IHC testing of human tonsil stained with CD6 antibody (3F7B5).

Formalin/paraffin human tonsil stained with CD6 antibody (3F7B5).

Species Reactivity	Human
Format	Purified
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1, kappa
Clone Name	3F7B5
Purity	Protein G affinity chromatography
Buffer	1X PBS, pH 7.4
Gene ID	923
Localization	Cell surface and cytoplasmic
Applications	Flow cytometry : 1-2ug/10^6 cells Immunofluorescence : 1-2ug/ml Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations	This CD6 antibody is available for research use only.
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Description

CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular scavenger receptor cysteine-rich (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. It shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. CD6 antibody can be used to deplete T cells from bone marrow transplants to prevent graft versus host disease.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.