CD5 Antibody Clone CD5/2418 / Microarray Specificity Validated Antibody [clone CD5/2419] (V7378)

CD5 Antibody Clone CD5/2418. Immunohistochemistry analysis of CD5 antibody staining in FFPE human tonsil tissue using a microarray specificity validated antibody. Strong membranous staining is observed in T lymphocytes within interfollicular regions, with dense labeling surrounding germinal centers while follicular B cell areas remain largely negative. The staining pattern highlights normal tonsillar architecture and supports selective detection of CD5-positive T cell populations, consistent with microarray specificity validation. Heat-induced epitope retrieval was performed in 10 mM citrate buffer, pH 6, for 10-20 minutes followed by cooling at room temperature prior to antibody incubation.

CD5 Antibody Clone CD5/2418. Protein microarray specificity analysis using a HuProt microarray containing more than 19,000 full-length human proteins demonstrates highly selective binding of the CD5/2418 monoclonal antibody to CD5. CD5 is identified as the top-ranked target with strong signal intensity (Z-score ~108.52) and clear separation from the next highest signals (S-score ~106.98), while all other proteins show minimal background binding. The Z-score reflects signal strength relative to the array mean, and the S-score represents the separation between the intended target and the next ranked protein, indicating relative specificity. These results support the high specificity profile of the CD5/2418 antibody for its intended target in proteome-scale screening.