CD5 Antibody Clone CD5/2419 / HuProt Validated Specificity Antibody [clone CD5/2418] (V7521)

CD5 Antibody Clone CD5/2419. Immunohistochemistry analysis of CD5 antibody staining in FFPE human tonsil tissue using a HuProt validated specificity antibody. Strong membranous staining is observed in T lymphocytes within interfollicular regions, with dense labeling surrounding germinal centers while follicular B cell areas remain largely negative. The staining pattern highlights normal tonsillar architecture and supports highly selective detection of CD5-positive T cell populations, consistent with proteome-scale HuProt validation. Heat-induced epitope retrieval was performed in 10 mM citrate buffer, pH 6, for 10-20 minutes followed by cooling at room temperature prior to antibody incubation.

CD5 Antibody Clone CD5/2419. Protein microarray specificity analysis using a HuProt microarray containing more than 19,000 full-length human proteins demonstrates highly selective binding of the CD5/2419 monoclonal antibody to CD5. CD5 is identified as the top-ranked target with strong signal intensity (Z-score ~124.74) and clear separation from the next highest signals (S-score ~122.87), while all other proteins show minimal background binding. The Z-score reflects signal strength relative to the array mean, and the S-score represents the separation between the intended target and the next ranked protein, indicating relative specificity. These results support the high specificity of the CD5/2419 antibody for its intended target in proteome-scale screening.

SDS-PAGE analysis of purified, BSA-free CD5 antibody (clone CD5/2418) as confirmation of integrity and purity.