- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
CD4 Antibody for FACS / Rodent T Cell Profiling Antibody (RQ5873)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ5873 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 449 |
| Availability | 1-3 business days |
| Species Reactivity | Mouse, Rat |
| Format | Antigen affinity purified |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Affinity purified |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide |
| UniProt | P05540 |
| Applications | Western Blot : 0.5-1ug/ml Immunohistochemistry : 1-2ug/ml Flow Cytometry : 1-3ug/million cells Direct ELISA : 0.1-0.5ug/ml |
| Limitations | This CD4 antibody is available for research use only. |
|
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CD4 molecule (CD4) is a cell surface glycoprotein expressed predominantly on T helper lymphocytes, with additional expression on monocytes, macrophages, and dendritic cells, where it functions as a co-receptor for antigen recognition through interaction with major histocompatibility complex class II molecules. CD4 Antibody for FACS is widely used in flow cytometry to identify CD4-positive immune cells, and is particularly valuable in rodent-based experimental systems where characterization of T cell populations is central to immunological research.
CD4 antibody, also referred to as T helper cell marker antibody or CD4 surface receptor antibody, is a fundamental reagent for flow cytometry analysis of immune cell populations. CD4 Antibody for FACS enables clear discrimination of CD4-positive lymphocytes from CD4-negative cells, supporting precise gating strategies and reproducible identification of helper T cell populations. In rodent immune studies, CD4 detection is essential for evaluating immune system composition, monitoring T cell responses, and investigating mechanisms of immune regulation in controlled model systems.
This CD4 Antibody for FACS is specifically positioned for rodent T cell profiling, where analysis of CD4-positive populations in mouse and rat samples is a routine component of preclinical research. Flow cytometry using CD4 antibodies allows researchers to quantify T helper cell populations, assess immune activation, and track dynamic changes in immune cell distribution during disease progression or therapeutic intervention. CD4 Antibody for FACS supports consistent detection of CD4-expressing cells, enabling reliable comparison of immune responses across experimental conditions.
CD4 is localized to the plasma membrane and plays a central role in T cell receptor signaling through its interaction with intracellular kinases such as Lck, amplifying antigen-specific activation pathways. Its expression defines a major functional subset of T lymphocytes responsible for coordinating adaptive immune responses. In flow cytometry assays, CD4 Antibody for FACS provides strong and specific surface staining, allowing accurate identification of CD4-positive T cells within heterogeneous leukocyte populations and supporting high-quality data generation in rodent immune profiling studies.
A rabbit polyclonal CD4 antibody can be used in flow cytometry to support detection of CD4-expressing T cells in experimental model systems. This antibody targets CD4 in research applications, enabling detailed characterization of immune cell populations and facilitating studies of T cell biology, immune regulation, and disease models in rodent systems.
A full range of CD4 antibody reagents for immunohistochemistry, western blot, and flow cytometry is available on our CD4 Antibody collection page.
Optimal dilution of the CD4 Antibody for FACS / Rodent T Cell Profiling Antibody should be determined by the researcher.
Recombinant rat protein (amino acids K28-I457) was used as the immunogen for the CD4 Antibody for FACS / Rodent T Cell Profiling Antibody.
After reconstitution, the CD4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
CD4 rodent FACS antibody, CD4 mouse rat antibody, CD4 T cell profiling antibody, CD4 flow cytometry antibody
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