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Home >> Antibodies >> CD3E Antibody / CD3 epsilon subunit

CD3E Antibody / CD3 epsilon subunit [clone MSVA-003R] (V6139)

  Catalog No Formulation Size Price (USD)  
Image V6139-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 654.50
Image
V6139-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
Microvalidated Recrabbitmono
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T-cell surface glycoprotein CD3 epsilon chain Rabbit Recombinant Monoclonal Antibody (MSVA-003R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MSVA-003R
UniProt P07766
Localization Cell membrane
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This CD3E/CD3 epsilon subunit antibody is available for research use only.
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Application Notes

1. Optimal dilution of the CD3E/CD3 epsilon subunit antibody should be determined by the researcher.

2. This CD3E/CD3 epsilon subunit antibody is recombinantly produced by expression in human HEK293 cells.

Immunogen

Synthetic peptide from human CD3e protein (around amino acids 20-40) (exact sequence is proprietary) was used as the immuongen for the CD3E/CD3 epsilon subunit antibody.

Storage

CD3E/CD3 epsilon subunit antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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