- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
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CD3 epsilon (CD3E) is a critical signaling component of the T-cell receptor (TCR) complex that is required for antigen recognition and downstream activation of T lymphocytes. CD3e Antibody for FACS / CD3 Epsilon Flow Cytometry Antibody enables fluorescence-based detection of CD3 Epsilon / CD3E, supporting detailed analysis of T-cell activation states and immune profiling in flow cytometry applications. CD3e antibody, also known as CD3 epsilon antibody or CD3E antibody, is widely used as a pan-T cell marker antibody and plays a central role in defining functional T-cell populations.
Within the TCR complex, CD3 epsilon associates with CD3 gamma, CD3 delta, and CD3 zeta chains to form a signaling platform that initiates intracellular activation cascades upon antigen engagement. This direct involvement in T-cell activation makes CD3 epsilon a key marker for studying immune signaling, functional responses, and T-cell behavior under different biological conditions. CD3e antibody reagents are therefore commonly used in experiments focused on immune activation, differentiation, and response to stimuli.
In flow cytometry, CD3e Antibody for FACS enables identification of T cells while also providing a framework for deeper functional analysis. Once CD3-positive populations are defined, additional markers can be applied to evaluate activation status, cytokine production, or differentiation state. This allows comprehensive immune profiling, where CD3 serves not only as a lineage marker but also as a gateway to functional characterization of T-cell subsets.
This CD3e Antibody for FACS is uniquely positioned for immune profiling applications where both accurate T-cell identification and high specificity are required. Clone C3e/2478 has demonstrated strong target recognition supported by protein microarray specificity data, indicating selective binding to CD3 epsilon with minimal cross-reactivity. This level of specificity is particularly important in multi-color flow cytometry panels, where non-specific binding can interfere with interpretation of closely related immune cell populations.
The inclusion of microarray specificity validation provides an additional layer of confidence in the antibody's performance, supporting its use in complex experimental designs where precise antigen recognition is critical. In flow cytometry, this translates to cleaner separation of CD3-positive populations and reduced background signal, enabling more accurate analysis of T-cell subsets and activation states.
CD3 epsilon flow cytometry antibody reagents are widely used in studies of infection, cancer, and immune-mediated diseases, where characterization of T-cell responses is essential. The ability to quantify and profile CD3-positive cells across conditions supports investigations into immune regulation and therapeutic response. By combining CD3 detection with additional markers, researchers can build detailed profiles of immune cell function and composition.
As a monoclonal antibody, clone C3e/2478 provides consistent epitope recognition and reproducible performance across experiments. CD3 epsilon Antibody for FACS using this clone supports high-confidence detection of T cells and enables robust immune profiling in flow cytometry-based research applications.
A full range of CD3e antibody reagents for immunohistochemistry, western blot, and flow cytometry is available on our CD3e Antibody collection page.
Optimal dilution of the CD3e Antibody for FACS / T Cell Activation and Immune Profiling Marker should be determined by the researcher.
A portion of amino acids 23-119 were used as the immunogen for the CD3e Antibody for FACS / T Cell Activation and Immune Profiling Marker.
Store the CD3e antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
CD3E antibody, CD3 activation marker antibody, CD3 immune profiling antibody, T cell activation antibody, CD3 signaling marker antibody
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