CD3e Antibody for FACS / T Cell Immunophenotyping Marker [clone C3e/1931] (V3683)

Description

CD3 epsilon (CD3E) is a membrane-associated component of the T-cell receptor (TCR) complex that is uniformly expressed on T lymphocytes and serves as a primary marker for defining T-cell populations. CD3e Antibody for FACS / CD3 Epsilon Flow Cytometry Antibody is specifically optimized for flow cytometry-based detection of CD3 Epsilon / CD3E, enabling precise identification and gating of T cells within complex cell mixtures. CD3e antibody, also known as CD3 epsilon antibody or CD3E antibody, is widely recognized as a pan-T cell marker antibody and is foundational to immunophenotyping workflows.

CD3 epsilon forms part of the CD3 signaling complex together with CD3 gamma, CD3 delta, and CD3 zeta chains, which associate with the TCR alpha-beta or gamma-delta heterodimer at the cell surface. This stable surface expression makes CD3 epsilon ideally suited for flow cytometry, where detection of membrane proteins enables rapid and quantitative discrimination of immune cell subsets. CD3e antibody reagents allow clear separation of CD3-positive T cells from CD3-negative populations such as B cells, NK cells, and myeloid cells.

In flow cytometry applications, CD3e Antibody for FACS functions as a primary gating marker, forming the backbone of T-cell identification strategies. CD3-positive events are first resolved within lymphocyte gates based on forward and side scatter, after which they are used to define total T-cell populations. This gating step is critical for downstream analysis, including subdivision into CD4-positive helper T cells and CD8-positive cytotoxic T cells. The reliability of CD3e antibody staining directly impacts the accuracy of these gating hierarchies and overall data interpretation.

This CD3e Antibody for FACS is uniquely positioned for immunophenotyping applications where clean population separation and reproducible gating are essential. Strong signal intensity and low non-specific background enable clear distinction between positive and negative populations, even in complex samples such as peripheral blood mononuclear cells or dissociated tissues. The ability to generate tight, well-resolved CD3-positive clusters supports consistent analysis across experiments and sample types.

In addition to defining T-cell populations, CD3 epsilon flow cytometry antibody reagents are widely used in studies examining immune composition and cell population dynamics. Quantification of CD3-positive cells provides insight into lymphocyte distribution and immune status, while also serving as a reference population for multi-parameter panels. This makes CD3e antibody reagents essential in both basic immunology and translational research settings.

As a rabbit polyclonal antibody, this CD3e Antibody for FACS provides multi-epitope recognition of CD3 epsilon, supporting robust signal generation and consistent detection across diverse sample preparations. CD3 epsilon Antibody for FACS serves as a core reagent for T-cell identification, enabling accurate gating and high-quality immunophenotyping in flow cytometry-based assays.

A full range of CD3e antibody reagents for immunohistochemistry, western blot, and flow cytometry is available on our CD3e Antibody collection page.

Application Notes

Optimal dilution of the CD3e Antibody for FACS / T Cell Immunophenotyping Marker should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A recombinant human partial protein corresponding to amino acids 23-119 from the human CD3 epsilon chain were used as the immunogen for the CD3e Antibody for FACS / T Cell Immunophenotyping Marker.

Storage

Store the CD3e antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

CD3E antibody, CD3 flow cytometry antibody, T cell immunophenotyping antibody, CD3 lymphocyte marker antibody, CD3 gating antibody