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Home >> Antibodies >> CD31 Antibody SPM122 / PECAM-1 Monoclonal

CD31 Antibody SPM122 / PECAM-1 Monoclonal [clone SPM122] (V8359)

  Catalog No Formulation Size Price (USD)  
Image V8359-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 559
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V8359-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 259
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V8359SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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CD31 Antibody SPM122 Immunohistochemistry Analysis. IHC staining of FFPE human angiosarcoma with CD31 antibody (clone SPM122). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free CD31 antibody (clone SPM122) as confirmation of integrity and purity.
Flow Cytometry testing of Jurkat cells. Black: cells alone; Green: isotype control; Red: PE-labeled PECAM antibody (SPM122).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name SPM122
Purity Protein G affinity chromatography
UniProt P16284
Localization Cell surface, cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Flow Cytometry : 1-3ug/million cells
Limitations This CD31 Antibody SPM122 is available for research use only.
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Description

CD31 Antibody SPM122 detects CD31 (PECAM-1), a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

For a Protein Microarray Validated monoclonal antibody with CD31 as the primary designation, see our CD31 Antibody PECAM1/3527 / PECAM-1 Monoclonal Antibody.

Application Notes

Optimal dilution of the CD31 Antibody SPM122 should be determined by the researcher.

Immunogen

A membrane preparation of a spleen from a patient with hairy cell leukemia was used as the immunogen for this CD31 Antibody SPM122.

Storage

Store the CD31 Antibody SPM122 at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

PECAM-1 antibody, PECAM1 antibody, CD31 antibody, Platelet Endothelial Cell Adhesion Molecule 1 antibody, EndoCAM antibody, PECA1 antibody, GPIIA antibody

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