CD31 Antibody Cocktail C31.3 + JC/70A / PECAM-1 Antibody Cocktail [clone C31.3 + JC/70A] (V8880)

CD31 Antibody Cocktail C31.3 + JC/70A detection of PECAM1 in THP-1 cells by WB. Western blot testing of human THP-1 cell lysate with CD31 Antibody Cocktail C31.3 + JC/70A. A strong predominant band is visible at approximately 120-130 kDa, within the expected 83-130 kDa range reported for CD31/PECAM1 depending on glycosylation. The higher apparent molecular weight relative to the underlying polypeptide is consistent with extensive glycosylation of mature PECAM1. The relatively discrete signal demonstrates recognition of the expected target by this PECAM-1 Antibody Cocktail in human cell lysate.

CD31 Antibody Cocktail C31.3 + JC/70A staining of colon carcinoma vasculature by IHC. Immunohistochemistry of FFPE human colon carcinoma sections using CD31 Antibody Cocktail C31.3 + JC/70A. Strong brown staining outlines vascular endothelial structures within the tumor and adjacent stromal regions, producing clearly defined vessel-associated labeling against the blue nuclear counterstain. The staining pattern is consistent with the characteristic endothelial localization of PECAM1 and demonstrates vascular detection with this PECAM-1 Antibody Cocktail. Heat-induced epitope retrieval was performed using pH 9 Tris-EDTA before testing.

CD31 Antibody Cocktail C31.3 + JC/70A staining of angiosarcoma by IHC. Immunohistochemistry of FFPE human angiosarcoma sections using CD31 Antibody Cocktail C31.3 + JC/70A. Strong brown staining highlights numerous irregular vascular channels and endothelial tumor cells throughout the section. The prominent membrane-associated labeling and complex vascular pattern are consistent with the endothelial differentiation characteristic of angiosarcoma. This image demonstrates robust recognition of PECAM1-positive vascular structures with the PECAM-1 Antibody Cocktail. Heat-induced epitope retrieval was performed using pH 9 Tris-EDTA before testing.