- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
CD25 Antibody 143-13 / Mouse Monoclonal [clone 143-13] (V2631)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V2631-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
|
|
V2631-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
|
|
V2631SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | 143-13 |
| Purity | Protein G affinity chromatography |
| UniProt | P01589 |
| Localization | Cell surface, Cytoplasmic |
| Applications | Functional Studies (order BSA/sodium Azide-free Format) : Flow Cytometry : 1-2ug/10^6 cells Immunofluorescence : 1-2ug/ml |
| Limitations | This CD25 Antibody 143-13 is available for research use only. |
|
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CD25 Antibody 143-13 recognizes a protein of 55 kDa, identified as CD25 (Workshop IV; Code A27). CD25 is expressed on activated T- and B-cells and activated monocytes/macrophages. With respect to lymphomas, CD25 is present on malignant cells of Hodgkins disease, HTLV-1+ adult T-cell leukemia, cutaneous T-cell lymphoma, and hair cell leukemia. Increased levels of soluble CD25 are observed in the leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 molecule alone appears to function as a low affinity receptor and associates with CD122 (IL-2R b-chain, p75) and CD132 (common g-chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. This mAb recognizes the epitope B, which is located at residue 3-104 of CD25 and can effectively block IL-2 binding to CD25.
For a monoclonal clone with IHC and protein microarray specificity validation, see our CD25 Antibody IL2RA/2395 / IL2RA Antibody page.
Optimal dilution of the CD25 Antibody 143-13 should be determined by the researcher.
Stimulated human leukocytes were used as the immunogen for the CD25 Antibody 143-13.
Store the CD25 Antibody 143-13 at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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