CD21 Antibody / Complement Receptor 2 CR2 Antibody [clone CR2/2754] (V3971)

CD21 Antibody Raji WB. Western blot analysis of Complement Receptor 2 / CD21 in human Raji cell lysate using mouse monoclonal CD21 antibody, clone CR2/2754. A band is detected at approximately 140–160 kDa, consistent with the predicted molecular weight of glycosylated CD21, which migrates above its theoretical mass of approximately 105 kDa due to extensive post-translational glycosylation characteristic of this B-cell surface receptor.

CD21 Antibody Microarray Validation. Protein microarray analysis of Complement Receptor 2 / CD21 using mouse monoclonal CD21 antibody, clone CR2/2754, across a HuProt(TM) array containing more than 19,000 full-length human proteins demonstrates highly selective binding to CD21 (CR2), with minimal reactivity toward non-target proteins. Signal intensity is strongly enriched for CD21 relative to all other proteins, supporting a high level of specificity. Z-score represents the strength of antibody binding signal expressed as standard deviations above the mean array signal, while S-score reflects the separation between the top-ranked target and the next highest signal, indicating relative target specificity. The observed signal profile confirms selective recognition of CD21 in a proteome-wide context.

CD21 Antibody Tonsil IHC. Immunohistochemistry of Complement Receptor 2 / CD21 in FFPE human tonsil tissue using mouse monoclonal CD21 antibody, clone CR2/2754. HRP-DAB brown staining highlights a dense reticular follicular dendritic cell network within germinal centers, with accompanying membranous staining of B lymphocytes, consistent with CD21 localization in B-cell-rich regions, while nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 6 10 mM citrate buffer for 10-20 min followed by cooling at RT prior to staining.

CD21 Antibody MOLT4 IF. Immunofluorescence analysis of Complement Receptor 2 / CD21 in PFA-fixed human MOLT4 cells using mouse monoclonal CD21 antibody, clone CR2/2754. Green fluorescence highlights CD21-positive cell surface and cytoplasmic signal, while nuclei are counterstained with Reddot (red).

CD21 Antibody MOLT4 FACS. Flow cytometry analysis of Complement Receptor 2 / CD21 in PFA-fixed human MOLT4 cells using CD21 antibody, clone CR2/2754. The blue histogram shows a clear rightward shift compared to the red isotype control, indicating detectable CD21 expression under the conditions tested.

SDS-PAGE analysis of purified, BSA-free CD21 antibody (clone CR2/2754) as confirmation of integrity and purity.

CD21 Antibody Germinal Center IHC. Immunohistochemistry of Complement Receptor 2 / CD21 in FFPE human tonsil tissue using mouse monoclonal CD21 antibody, clone CR2/2754. HRP-DAB brown staining highlights dense membranous labeling of B lymphocytes within germinal center regions, with an underlying reticular pattern corresponding to follicular dendritic cell networks, while nuclei are counterstained blue. Inset: PBS was used in place of primary antibody as a negative control to confirm specificity of staining. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling prior to staining.