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Home >> Antibodies >> CD2 Antibody

CD2 Antibody [clone UMCD2] (V2030)

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  Catalog No Formulation Size Price (USD)  
Image V2030-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 539
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V2030-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 249
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V2030SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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Flow cytometry testing of human MOLT-4 cells with CD2 antibody (clone UMCD2); Red=isotype control, Blue= CD2 antibody.
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Flow cytometry testing of human MOLT-4 cells with CD2 antibody (clone UMCD2); Red=isotype control, Blue= CD2 antibody.
Western blot testing of human Jurkat cells with CD2 antibody (clone UMCD2). Expected molecular weight: 38-50 kDa depending on level of glycosylation.
SDS-PAGE analysis of purified, BSA-free CD2 antibody (clone UMCD2) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name UMCD2
Purity Protein G purified antibody
Gene ID 914
Localization Cell surface
Applications Functional studies : order BSA/sodium azide-free format
Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Western blot : 2-4ug/ml
Limitations This CD2 antibody is available for research use only.
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Description

CD2 interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate cell adhesion. CD2/CD58 binding can enhance antigen-specific Tcell activation. CD2 is a transmembrane glycoprotein that is expressed on peripheral blood T lymphocytes, NK cells and thymocytes. CD58 is a heavily glycosylated protein with a broad tissue distribution in hematopoietic and other cells, including endothelium. Interaction between CD2 and its counter receptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby facilitating communication between helper T lymphocytes and antigen-presenting cells, as well as between cytolytic effectors and target cells.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondary antibody and substrates may require the CD2 antibody to be titered up or down for optimal performance.

Immunogen

Human thymocytes/Sezary T cells were used as the immunogen for this CD2 antibody.

Storage

Store the CD2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Erythrocyte receptor; LFA-2; LFA-3 receptor; Ly-37; Lymphocyte function antigen 2; Rosette receptor; SRBC; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5; T11

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