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Home >> Antibodies >> CD2 Antibody

CD2 Antibody (R30833)

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  Catalog No Formulation Size Price (USD)  
Image R30833 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of CD2 antibody and Lane 1: rat spleen; 2: mouse NIH3T3 cell lysate
Western blot testing of CD2 antibody and Lane 1: rat spleen; 2: mouse NIH3T3 cell lysate
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P08920
Applications Western blot : 0.5-1ug/ml
Limitations This CD2 antibody is available for research use only.
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Description

Cluster of differentiation 2 is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that the protein is the erythrocyte receptor or is closely associated with it. It is one of the earliest T-cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes. Due to its structural characteristics, CD2 is a member of the immunoglobulin superfamily; it possesses two immunoglobulin-like domains in its extracellular portion. CD2 interacts with other adhesion molecules, such as lymphocyte function-associated antigen-3(LFA-3/CD58) in humans, or CD48 in rodents, which are expressed on the surfaces of other cells. With the use of transgenic mice, such an LCR was identified within the 3-prime flanking region of the human gene.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the CD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the middle region of mouse CD2 (FIFCICKRRKRNRRRKDEE) was used as the immunogen for this CD2 antibody.

Storage

After reconstitution, the CD2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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