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Home >> Antibodies >> CD1b Antibody

CD1b Antibody [clone RIV12] (V2028)

  Catalog No Formulation Size Price (USD)  
Image V2028-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2028-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2028SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
FACS staining of human PBMCs using CD1b antibody (RIV12).
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name RIV12
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 910
Localization Cell surface, cytoplasmic
Applications ELISA (order BSA/sodium azide-free format for coating) :
Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 0.5-1ug/ml
Limitations This CD1b antibody is available for research use only.
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Description

This antibody recognizes CD1b, a 44kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes, and T acute lymphoblastic leukemia cells. The CD1 multigene family encodes five forms of the CD1 T-cell surface glycoprotein in human, designated CD1A, 1B, 1C, 1D and 1E. CD1, a type 1 membrane protein, has structural similarity to the MHC class I antigen and has been shown to present lipid antigens for recognition by T lymphocytes. Constitutive endocytosis of CD1b molecules and the differential sorting of MHC class II from lysosomes separate peptide- and lipid antigen-presenting molecules during dendritic cell maturation. It is also expressed in interdigitating cells.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD1b antibody to be titered up or down for optimal performance.

Immunogen

Human peripheral lymphocytes were used as the immunogen for this CD1b antibody.

Storage

Store the CD1b antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

hTa1 thymocyte antigen; Ly38; T6/Leu6

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