CD162 Antibody | P-Selectin Glycoprotein Ligand 1

	Catalog No	Formulation	Size	Price (USD)
	V4316-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	100 ug	559
Formulation
	V4316-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	20 ug	259
Formulation
	V4316SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	559
Formulation

CD162 Antibody PSGL1/8135R in Human Tonsil IHC. Immunohistochemistry staining of FFPE human tonsil tissue with CD162 Antibody / P-Selectin Glycoprotein Ligand 1 Antibody, clone PSGL1/8135R, at 1-2 ug/ml for 30 min at RT. HIER was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 min followed by cooling before testing. The observed staining demonstrates CD162 expression in human tonsil tissue.

CD162 Antibody PSGL1/8135R in Human Tissue Panel IHC. Immunohistochemistry analysis of FFPE human tissues with CD162 Antibody / P-Selectin Glycoprotein Ligand 1 Antibody, clone PSGL1/8135R, at 1-2 ug/ml for 30 min at RT. Samples shown are: 1) mammary carcinoma, 2) transverse muscle, 3) tonsil and 4) tonsil. HIER was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 min followed by cooling before testing. PSGL-1 staining demonstrates CD162 detection across the human tissue samples.

CD162 Antibody PSGL1/8135R in Human Lung IHC. Immunohistochemistry staining of FFPE human lung tissue with CD162 Antibody / P-Selectin Glycoprotein Ligand 1 Antibody, clone PSGL1/8135R, at 1-2 ug/ml for 30 min at RT. HIER was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 min followed by cooling before testing. The observed staining demonstrates PSGL-1 expression in human lung tissue.

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CD162 Antibody PSGL1/8135R in Human Tonsil IHC. Immunohistochemistry staining of FFPE human tonsil tissue with CD162 Antibody / P-Selectin Glycoprotein Ligand 1 Antibody, clone PSGL1/8135R, at 1-2 ug/ml for 30 min at RT. HIER was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 min followed by cooling before testing. The observed staining demonstrates CD162 expression in human tonsil tissue.

CD162 Antibody PSGL1/8135R in Human Tissue Panel IHC. Immunohistochemistry analysis of FFPE human tissues with CD162 Antibody / P-Selectin Glycoprotein Ligand 1 Antibody, clone PSGL1/8135R, at 1-2 ug/ml for 30 min at RT. Samples shown are: 1) mammary carcinoma, 2) transverse muscle, 3) tonsil and 4) tonsil. HIER was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 min followed by cooling before testing. PSGL-1 staining demonstrates CD162 detection across the human tissue samples.

CD162 Antibody PSGL1/8135R in Human Lung IHC. Immunohistochemistry staining of FFPE human lung tissue with CD162 Antibody / P-Selectin Glycoprotein Ligand 1 Antibody, clone PSGL1/8135R, at 1-2 ug/ml for 30 min at RT. HIER was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 min followed by cooling before testing. The observed staining demonstrates PSGL-1 expression in human lung tissue.

Availability	1-3 business days
Species Reactivity	Human
Format	Purified
Host	Rabbit
Clonality	Recombinant Rabbit Monoclonal
Isotype	Rabbit IgG, kappa
Clone Name	PSGL1/8135R
Purity	Protein A/G affinity
UniProt	Q14242
Localization	Cell surface
Applications	Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations	This CD162 Antibody / P-Selectin Glycoprotein Ligand 1 Antibody is available for research use only.
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Description

CD162 Antibody / P-Selectin Glycoprotein Ligand 1 Antibody detects CD162, a cell-surface glycoprotein encoded by the SELPLG gene and widely known as PSGL-1. CD162 functions as a high-affinity counter-receptor for selectins and is expressed on myeloid cells, lymphocytes and other hematopoietic cell populations. Through interactions with P-, E- and L-selectin, PSGL-1 participates in cell adhesion and the recruitment of leukocytes from the circulation into tissues during inflammatory and immune responses.

PSGL-1 is a mucin-like transmembrane glycoprotein whose extracellular region is extensively glycosylated. Functional selectin binding depends on specific post-translational modifications, including tyrosine sulfation and formation of sialyl Lewis x-containing O-glycans. These modifications help generate the high-affinity binding site required for efficient interaction with selectins. PSGL-1 can also form homodimers and contains a cytoplasmic region that connects the receptor with intracellular structural and signaling proteins.

P-Selectin Glycoprotein Ligand 1 is particularly important during the early stages of leukocyte recruitment. Binding between PSGL-1 on circulating leukocytes and selectins on activated endothelial cells or platelets promotes leukocyte tethering and rolling under vascular flow. These initial adhesive interactions help position immune cells for subsequent firm adhesion and migration across the vascular endothelium into sites of inflammation. PSGL-1-mediated interactions are therefore studied in neutrophil, monocyte and lymphocyte trafficking as well as broader mechanisms regulating inflammatory cell recruitment.

CD162 also has functions extending beyond its classical role as an adhesion molecule. Selectin engagement can influence intracellular signaling and immune-cell behavior, while PSGL-1 has been investigated in adaptive immunity, infection and regulation of T-cell responses. Expression and functional glycosylation of PSGL-1 can vary according to cell type and activation state, adding another level of regulation to selectin-dependent leukocyte trafficking. These properties make CD162 relevant to studies of inflammation, immune-cell migration and communication between circulating leukocytes and vascular tissues.

Clone PSGL1/8135R is a recombinant rabbit monoclonal antibody generated against full-length recombinant human protein. It has been evaluated by immunohistochemistry in FFPE human tissues, including tonsil, mammary carcinoma, transverse muscle and lung. Recombinant production provides a defined antibody sequence and supports consistent performance across manufacturing lots. NSJ Bioreagents supplies antibodies for immunology, inflammation and cell adhesion research. A CD162 Antibody can be used to investigate PSGL-1 expression, selectin interactions, leukocyte trafficking and mechanisms regulating inflammatory cell recruitment.

For related antibodies targeting CD molecules involved in immune-cell recognition, adhesion and signaling, explore our CD Antibodies page.

Application Notes

Optimal dilution of the CD162 Antibody / P-Selectin Glycoprotein Ligand 1 Antibody should be determined by the researcher.

Immunogen

Recombinant full-length human protein was used as the immunogen for the CD162 antibody.

Storage

Aliquot the CD162 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Alternate Names

CD162 antibody, P-Selectin Glycoprotein Ligand 1 antibody, PSGL-1 antibody, SELPLG antibody, PSGL1 antibody

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CD162 Antibody / P-Selectin Glycoprotein Ligand 1 Antibody [clone PSGL1/8135R] (V4316)

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Area of Research

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NSJ Bioreagents