CD14 Antibody LPSR/2397 / Monocyte Differentiation Antigen Antibody [clone LPSR/2397] (V3753)

CD14 Antibody LPSR/2397 by IHC in Human Tonsil. Immunohistochemistry staining of FFPE human tonsil tissue using mouse monoclonal CD14 antibody LPSR/2397. Brown immunoreactivity highlights numerous cells distributed through the tissue, with particularly prominent staining along cellular boundaries and branching processes. The staining is predominantly membranous and peripheral cytoplasmic, contrasting with the lighter hematoxylin-counterstained background. This pattern is consistent with CD14 expression in monocyte/macrophage-associated populations within tonsillar tissue. The Monocyte Differentiation Antigen CD14 participates in bacterial lipopolysaccharide recognition and innate immune signaling. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 minutes, followed by cooling at room temperature for 20 minutes.

CD14 Antibody LPSR/2397 by IHC in Human Lymph Node. Immunohistochemistry staining of FFPE human lymph node tissue using mouse monoclonal CD14 antibody LPSR/2397. Strong brown immunoreactivity is concentrated within two prominent rounded cellular regions, while the surrounding lymphoid tissue exhibits considerably weaker staining. Positive cells display membranous and cytoplasmic staining, producing a heterogeneous distribution across the section. The image demonstrates localized detection of CD14-associated immunoreactivity in human lymphoid tissue. CD14 is also known as the Monocyte Differentiation Antigen and is expressed by monocytes, macrophages, and selected other myeloid populations. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 minutes, followed by cooling at room temperature for 20 minutes.

CD14 Antibody LPSR/2397 by Protein Microarray Specificity Analysis. HuProt protein microarray analysis of mouse monoclonal CD14 antibody LPSR/2397 against more than 19,000 full-length human proteins. The specificity graph identifies CD14 as the highest-ranked target, with a Z-score of approximately 110.24. The next-ranked proteins exhibit substantially lower signals, supporting preferential recognition of CD14 under the assay conditions. Z-scores represent fluorescence signals relative to the distribution of signals across the array, while the S-score measures separation between ranked protein signals. These results provide protein microarray evidence supporting the specificity of clone LPSR/2397 for the Monocyte Differentiation Antigen CD14.

CD14 Antibody LPSR/2397 by SDS-PAGE Showing Antibody Integrity and Purity. SDS-PAGE analysis of purified, BSA-free mouse monoclonal CD14 antibody LPSR/2397, with approximately 2 ug of antibody loaded under reducing (R) and non-reducing (NR) conditions. The non-reduced sample shows a prominent band near 150 kDa, consistent with intact immunoglobulin. Under reducing conditions, bands are visible near 50 kDa and 25 kDa, corresponding to the immunoglobulin heavy and light chains, respectively. This experiment confirms the expected molecular composition of the purified antibody preparation and serves as a quality-control assessment rather than direct validation of CD14 recognition.

CD14 Antibody LPSR/2397 by WB in Human A549 Cells. Western blot analysis of human A549 cell lysate using mouse monoclonal CD14 antibody LPSR/2397. A prominent immunoreactive band is detected near 55 kDa, consistent with the expected molecular weight of mature CD14. The signal is relatively discrete, with limited additional reactivity in the displayed molecular weight range. CD14 is a glycosylphosphatidylinositol-anchored glycoprotein with a predicted molecular weight of approximately 40 kDa before post-translational modification. Glycosylation can increase its apparent molecular weight to approximately 50-55 kDa, providing a plausible explanation for the observed migration. This result demonstrates recognition of a protein consistent with the Monocyte Differentiation Antigen CD14 in human A549 cells.

CD14 Antibody LPSR/2397 by WB in Human THP-1 Cells. Western blot analysis of human THP-1 monocytic cell lysate using mouse monoclonal CD14 antibody LPSR/2397. A prominent immunoreactive signal is observed around 50-65 kDa, with the strongest intensity near 55 kDa. The band appears broader than the corresponding A549 signal, suggesting heterogeneity in electrophoretic mobility. CD14 is a glycosylated cell surface protein whose apparent molecular weight can vary with post-translational modification. Differences in glycosylation may contribute to the broader signal, although the experiment does not establish the cause. THP-1 cells are commonly used to investigate monocyte differentiation and innate immune responses, making them a relevant model for studying CD14 expression.