CD14 Monoclonal Antibody LPSR/2386 / LPS Receptor Antibody [clone LPSR/2386] (V3763)

CD14 Monoclonal Antibody LPSR/2386 by FACS in Human A549 Cells. Flow cytometry analysis of human A549 cells using mouse monoclonal CD14 antibody LPSR/2386. The antibody-stained population (blue) exhibits a rightward fluorescence shift relative to the isotype control (red), indicating increased CD14-associated fluorescence. The two distributions overlap considerably, although their peaks are distinguishable. This experiment supports detection of CD14 in the tested A549 cells and demonstrates the performance of clone LPSR/2386 in flow cytometry. CD14, also known as the LPS receptor, participates in bacterial endotoxin recognition and innate immune signaling.

CD14 Monoclonal Antibody LPSR/2386 by IHC in Human Tonsil. Immunohistochemistry staining of FFPE human tonsil tissue using mouse monoclonal CD14 antibody LPSR/2386. Strong brown immunoreactivity highlights numerous cells within a densely populated region of the tissue. The staining outlines individual cells and produces a prominent membranous pattern, with additional cytoplasmic reactivity. Surrounding areas contain more weakly stained cells, providing contrast with the CD14-positive population. The distribution is consistent with expression of the LPS Receptor in myeloid cells within lymphoid tissue. Heat-induced epitope retrieval was performed by boiling sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 minutes, followed by cooling at room temperature for 20 minutes.

CD14 Monoclonal Antibody LPSR/2386 by IHC in Human Lymph Node. Immunohistochemistry staining of FFPE human lymph node tissue using mouse monoclonal CD14 antibody LPSR/2386. Brown immunoreactivity is concentrated within a rounded cellular region, where numerous positive cells exhibit membranous and peripheral cytoplasmic staining. The surrounding lymphoid tissue shows substantially weaker staining, emphasizing the localized distribution of CD14-positive cells. This pattern is consistent with expression of the LPS Receptor in monocyte/macrophage populations within lymphoid tissue. Heat-induced epitope retrieval was performed by boiling sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 minutes, followed by cooling at room temperature for 20 minutes.

CD14 Monoclonal Antibody LPSR/2386 by WB in Human A549 Cells. Western blot analysis of human A549 cell lysate using mouse monoclonal CD14 antibody LPSR/2386. A prominent immunoreactive band is detected near 55 kDa, consistent with the expected molecular weight of mature CD14. The datasheet specifies an expected molecular weight range of 40-55 kDa, depending on glycosylation. The observed band is relatively discrete, with limited additional reactivity in the displayed molecular weight range. CD14 is a glycosylphosphatidylinositol-anchored glycoprotein that participates in bacterial lipopolysaccharide recognition. Migration near the upper end of the expected range may reflect glycosylation, although the modification status was not independently established in this experiment.

CD14 Monoclonal Antibody LPSR/2386 by Protein Microarray Specificity Analysis. HuProt protein microarray analysis of mouse monoclonal CD14 antibody LPSR/2386 against more than 19,000 full-length human proteins. CD14 produces the highest-ranked signal, with a Z-score of approximately 110.9, while other proteins generate substantially lower signals. The strong separation between CD14 and the remaining proteins supports preferential recognition of the intended target under the assay conditions. The Z-score represents signal intensity relative to the distribution of array signals, while the S-score reflects the separation between ranked protein signals. These results provide protein microarray evidence supporting the specificity of clone LPSR/2386 for CD14.

CD14 Monoclonal Antibody LPSR/2386 by SDS-PAGE Showing Antibody Integrity and Purity. SDS-PAGE analysis of purified, BSA-free mouse monoclonal CD14 antibody LPSR/2386, with approximately 2 ug of antibody loaded per lane. The non-reduced (NR) sample shows a prominent band near 150 kDa, consistent with intact immunoglobulin. Under reducing (R) conditions, bands are visible near 50 kDa and 25 kDa, corresponding to immunoglobulin heavy and light chains. This analysis demonstrates the expected molecular composition of the purified antibody preparation and serves as a quality-control assessment rather than direct validation of CD14 recognition.

CD14 Monoclonal Antibody LPSR/2386 by WB in Human THP-1 Cells. Western blot analysis of human THP-1 monocytic cell lysate using mouse monoclonal CD14 antibody LPSR/2386. A prominent, relatively broad immunoreactive signal is observed around 45-60 kDa, with the strongest intensity near 50-55 kDa. This migration is consistent with the expected 40-55 kDa molecular weight range of CD14, allowing for variation associated with glycosylation. The broad signal may reflect heterogeneous glycosylation, although this has not been independently established. THP-1 cells are commonly used to investigate monocyte differentiation and innate immune responses, making them a relevant experimental model for evaluating CD14 expression.