CD14 Antibody LPSR/2385 / Macrophage Marker Antibody [clone LPSR/2385] (V3758)

CD14 Antibody LPSR/2385 by IHC in Human Lymph Node. Immunohistochemistry staining of FFPE human lymph node tissue using mouse monoclonal CD14 antibody LPSR/2385. Strong brown immunoreactivity is visible in numerous cells distributed throughout the tissue, with prominent membranous and cytoplasmic staining. The positive cells exhibit irregular outlines and are interspersed with lighter, weakly stained regions. This pattern is consistent with CD14 expression in monocyte/macrophage populations within lymphoid tissue. Heat-induced epitope retrieval was performed by boiling sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 minutes, followed by cooling at room temperature for 20 minutes.

CD14 Antibody LPSR/2385 by IHC in Human Tonsil. Immunohistochemistry staining of FFPE human tonsil tissue using mouse monoclonal CD14 antibody LPSR/2385. Prominent brown staining is concentrated in a rounded cellular region, with positive cells displaying predominantly membranous and peripheral cytoplasmic immunoreactivity. Surrounding lymphoid tissue exhibits substantially weaker staining, creating clear contrast between CD14-positive and less-reactive areas. The distribution is consistent with CD14 expression in myeloid cell populations within tonsillar tissue. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 minutes, followed by cooling at room temperature for 20 minutes.

CD14 Antibody LPSR/2385 by WB in Human A549 Cells. Western blot analysis of human A549 cell lysate using mouse monoclonal CD14 antibody LPSR/2385. A prominent immunoreactive band is observed near 55 kDa, consistent with the expected molecular weight of mature CD14. The predicted molecular weight range is approximately 40-55 kDa, depending on glycosylation. CD14 is a glycosylphosphatidylinositol-anchored glycoprotein that participates in bacterial lipopolysaccharide recognition and innate immune signaling. The observed migration near the upper end of the expected range may reflect glycosylation, although the modification status was not independently established in this experiment. The relatively discrete band demonstrates recognition of a protein consistent with CD14 in human A549 lysate.

CD14 Antibody LPSR/2385 by FACS in Human A549 Cells. Flow cytometry analysis of human A549 cells using mouse monoclonal CD14 antibody LPSR/2385. The antibody-stained population (blue) exhibits a clear rightward fluorescence shift relative to the isotype control (red), indicating detectable CD14-associated fluorescence. The histogram shows separation between the two populations, supporting antibody recognition of CD14 in the tested cells. This experiment provides flow cytometry validation for clone LPSR/2385 and demonstrates its ability to detect the macrophage-associated lipopolysaccharide receptor under the experimental conditions shown.

CD14 Antibody LPSR/2385 by Protein Microarray Specificity Analysis. HuProt protein microarray analysis of mouse monoclonal CD14 antibody LPSR/2385 against more than 19,000 full-length human proteins. The specificity plot identifies CD14 as the highest-ranked protein target, with a Z-score of approximately 97.6. Other proteins produce substantially lower signals, indicating preferential recognition of CD14 under the assay conditions. The Z-score represents signal intensity relative to the distribution of array signals, while the S-score reflects separation between the intended target and other detected proteins. These results provide protein microarray evidence supporting the target specificity of clone LPSR/2385.

CD14 Antibody LPSR/2385 by SDS-PAGE Showing Antibody Integrity and Purity. SDS-PAGE analysis of purified, BSA-free mouse monoclonal CD14 antibody LPSR/2385, with approximately 2 ug of antibody loaded per lane. The non-reduced (NR) sample shows a prominent band near 150 kDa, consistent with intact immunoglobulin. Under reducing (R) conditions, the antibody separates into bands near 50 kDa and 25 kDa, corresponding to the immunoglobulin heavy and light chains. This experiment demonstrates the expected molecular composition of the purified antibody preparation and serves as a quality-control assessment rather than direct validation of CD14 recognition.

CD14 Antibody LPSR/2385 by WB in Human THP-1 Cells. Western blot analysis of human THP-1 monocytic cell lysate using mouse monoclonal CD14 antibody LPSR/2385. A strong, relatively broad immunoreactive signal is observed around 40-60 kDa, with the most intense staining near 45-55 kDa. This migration range is consistent with the expected molecular weight of CD14, which can vary depending on glycosylation. THP-1 cells are commonly used to investigate monocyte differentiation and innate immune signaling, making them a relevant model for evaluating CD14 expression. The broad signal may reflect glycosylation heterogeneity, although this explanation has not been experimentally confirmed by the blot. The result supports recognition of CD14-associated protein in human monocytic cells.