IL7R Antibody IL7R/2751 / CD127 Monoclonal Antibody [clone IL7R/2751] (V7294)

IL7R Antibody IL7R/2751 Detects CD127 in Human Cell Lines WB. Western blot analysis of human cell lysates using IL7R Antibody (clone IL7R/2751) shows strong, discrete bands at approximately 55-60 kDa. Lane 1: K562 cells; lane 2: Jurkat T lymphocytes; lane 3: HeLa cervical cancer cells; lane 4: Ramos B lymphocytes. The closely aligned major bands across all four samples are consistent with detection of glycosylated IL7R/CD127. This CD127 Monoclonal Antibody produces a clean, predominantly single-band pattern with little visible background.

IL7R Antibody IL7R/2751 Protein Microarray Validated. HuProt protein microarray analysis of IL7R Antibody (clone IL7R/2751) against more than 19,000 full-length human proteins identifies CD127/IL7R as the dominant target, with a Z-score of 100.8 and S-score of 99.1. The Z-score represents signal strength in standard deviations above the array mean, while the S-score measures separation from the next-ranked protein. The exceptionally large separation from the secondary signals provides strong evidence of preferential IL7R recognition by this CD127 Monoclonal Antibody under the tested microarray conditions.

IL7R Antibody IL7R/2751 Purity and Integrity by SDS-PAGE. SDS-PAGE analysis of 2 ug purified, BSA-free IL7R Antibody (clone IL7R/2751) shows the intact antibody migrating near 150 kDa under non-reducing conditions. Under reducing conditions, the expected immunoglobulin heavy and light chains are visible at approximately 50 kDa and 25 kDa, respectively. The observed migration pattern supports the integrity and purity of this CD127 Monoclonal Antibody preparation.