CD123 Antibody IL3RA/1531 / IL3RA Monoclonal Antibody [clone IL3RA/1531] (V7291)

CD123 Antibody IL3RA/1531 in Human Lymph Node IHC. Immunohistochemistry of FFPE human lymph node tissue using CD123 Antibody clone IL3RA/1531 demonstrates strong staining in a distinct population of cells, with prominent membranous and cytoplasmic signal contrasting with the largely negative surrounding lymphoid cells. HIER was performed by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9 for 10-20 min followed by cooling. The selective staining demonstrates detection of IL3RA by this IL3RA Monoclonal Antibody in human lymph node.

CD123 Antibody IL3RA/1531 in Human Tonsil IHC. Immunohistochemistry of FFPE human tonsil tissue using CD123 Antibody clone IL3RA/1531 demonstrates distinct staining of scattered cells distributed throughout the lymphoid tissue, while most surrounding lymphocytes remain comparatively unstained. HIER was performed by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9 for 10-20 min followed by cooling. The discrete positive-cell population demonstrates detection of IL3RA by this IL3RA Monoclonal Antibody in human tonsil.

CD123 Antibody IL3RA/1531 in Human Gastric Carcinoma IHC. Immunohistochemistry of FFPE human gastric carcinoma tissue using CD123 Antibody clone IL3RA/1531 demonstrates strong staining across a substantial population of tumor cells, with prominent membranous and cytoplasmic signal and comparatively little staining in intervening stromal areas. HIER was performed by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9 for 10-20 min followed by cooling. The robust tumor-cell staining demonstrates detection of IL3RA by this IL3RA Monoclonal Antibody in human gastric carcinoma.

CD123 Antibody IL3RA/1531 Protein Microarray Validated. HuProt protein microarray analysis of CD123 Antibody clone IL3RA/1531 against more than 19,000 full-length human proteins identifies CD123 as the dominant target, with a Z-score of 134.65 and an S-score of 109.49. The Z-score measures signal strength in standard deviations above the mean array signal, while the S-score measures separation between the intended target and the next highest-ranking protein. The exceptionally large separation of CD123 from the remaining proteins provides strong specificity evidence for this IL3RA Monoclonal Antibody.

CD123 Antibody IL3RA/1531 Purity by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free CD123 Antibody clone IL3RA/1531 with a 2 ug load demonstrates an intact immunoglobulin species at approximately 150 kDa under non-reducing conditions. Under reducing conditions, the antibody resolves into prominent heavy- and light-chain bands at approximately 50 kDa and 25 kDa, respectively. The clean banding pattern provides confirmation of the integrity and purity of this IL3RA Monoclonal Antibody preparation.