CBLIF Antibody / Cobalamin Binding Intrinsic Factor Antibody (RQ7732)

CBLIF Antibody Human U2OS IF. Immunofluorescent staining of U2OS human osteosarcoma cells using CBLIF Antibody (red) following enzyme-mediated antigen retrieval and DAPI nuclear counterstaining (blue). CBLIF displays predominantly cytoplasmic staining with a granular distribution, consistent with its synthesis as a secreted glycoprotein involved in vitamin B12 binding and transport. This result demonstrates the utility of CBLIF Antibody, a Cobalamin Binding Intrinsic Factor Antibody, for investigating intrinsic factor expression, vitamin B12 absorption and gastrointestinal protein biology in cultured human cells.

CBLIF Antibody Human and Mouse Stomach WB. Western blot analysis of CBLIF Antibody using lane 1: human HGC-27 cells and lane 2: mouse stomach tissue lysate. A specific band is detected at approximately 45-50 kDa in both samples, consistent with the predicted molecular weight of CBLIF, with the slightly higher apparent molecular weight reflecting expected glycosylation of this secreted glycoprotein. These results demonstrate cross-species detection of endogenous CBLIF and support the use of CBLIF Antibody, a Cobalamin Binding Intrinsic Factor Antibody, for studies of vitamin B12 absorption, gastric physiology and intrinsic factor biology.

CBLIF Antibody Human U937 FACS. Flow cytometry analysis of human U937 cells stained with CBLIF Antibody at 1 ug/10^6 cells following blocking with goat serum. The blue histogram (CBLIF) shows a clear rightward shift compared with the isotype control (green) and unstained cells (red), demonstrating specific detection of endogenous CBLIF. These results support the use of CBLIF Antibody, a Cobalamin Binding Intrinsic Factor Antibody, for analyzing intrinsic factor expression and vitamin B12 transport protein biology by flow cytometry.

CBLIF Antibody Human Stomach IHC. Immunohistochemical staining of FFPE human stomach tissue using CBLIF Antibody at 2 ug/ml with HRP/DAB detection following heat-induced epitope retrieval in pH8 EDTA buffer. Strong cytoplasmic staining is observed in gastric parietal cells, consistent with the expected expression of cobalamin binding intrinsic factor in the gastric mucosa. This staining pattern demonstrates the utility of CBLIF Antibody, a Cobalamin Binding Intrinsic Factor Antibody, for investigating vitamin B12 absorption, gastric physiology and intrinsic factor expression in human tissues.