- Tel: 858.663.9055
- Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Related Products
|
Caspase-3, also known as YAMA, CPP32 or APOPAIN, is a member of the cysteine-aspartic acid protease family known to interact with family members -8 and -9. It is encoded by the CASP3 gene. Tiso et al.(1996) used radiation hybrid mapping to localize the gene to human chromosome 4q33-q35.1. Fernandes-Alnemri et al.(1994) found that overexpression of CASP3 in insect cells induced apoptosis. Coexpression of the two CASP3 subunits in insect cells also resulted in apoptosis. Tewari et al.(1995) showed that purified human CASP1 cleaved the CASP3 proenzyme into a proteolytically active form and that activated Caspase-3 cleaved PARP into the 85-kD apoptotic form.
The stated application concentrations are suggested starting points. Titration of the Caspase-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
An amino acid sequence from the C-terminus of the human protein (CAMLKQYADKLEFMHIL) was used as the immunogen for this Caspase-3 antibody. This amino acid sequence is from the small subunit.
After reconstitution, the Caspase-3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.