Carboxypeptidase A1 Antibody Microarray Specificity Validated / CPA1 Antibody [clone CPA1/2714] (V7404)

Carboxypeptidase A1 Antibody Pancreas IHC. Immunohistochemistry of Carboxypeptidase A1 / CPA1 in FFPE human pancreas tissue using mouse monoclonal Carboxypeptidase A1 antibody, clone CPA1/2714. Strong HRP-DAB brown cytoplasmic staining highlights pancreatic acinar cells, with clear negative islet regions providing internal contrast, consistent with the highly restricted expression pattern of CPA1 and supporting the specificity profile of this antibody, while nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 6 10 mM citrate buffer for 10-20 min followed by cooling at RT prior to staining.

Carboxypeptidase A1 Antibody Human Pancreas Tissue Immunohistochemistry. IHC staining of Carboxypeptidase A1 / CPA1 in FFPE human pancreas tissue using mouse monoclonal Carboxypeptidase A1 antibody, clone CPA1/2714. Strong HRP-DAB brown cytoplasmic staining highlights pancreatic acinar cells, with clear negative islet regions providing internal contrast, consistent with the highly restricted expression pattern of CPA1 and supporting the specificity profile of this antibody, while nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 6 10 mM citrate buffer for 10-20 min followed by cooling at RT prior to staining.

Carboxypeptidase A1 Antibody Microarray Validation. Protein microarray analysis of Carboxypeptidase A1 / CPA1 using mouse monoclonal Carboxypeptidase A1 antibody, clone CPA1/2714, demonstrates selective binding to CPA1 among a large panel of human proteins. Signal intensity is strongly enriched for the target protein, while non-target proteins show minimal reactivity, supporting a high level of specificity. Z-score represents the strength of antibody binding signal relative to the array background, expressed as standard deviations above the mean, while S-score reflects the separation between the top-ranked target and the next highest signal, indicating relative target specificity. The observed signal profile supports selective recognition of CPA1 in a proteome-wide context.

SDS-PAGE analysis of purified, BSA-free Carboxypeptidase A1 antibody (clone CPA1/2714) as confirmation of integrity and purity.

Carboxypeptidase A1 Antibody Pancreas WB. Western blot analysis of Carboxypeptidase A1 / CPA1 in human pancreas tissue lysate using mouse monoclonal Carboxypeptidase A1 antibody, clone CPA1/2714. A band is detected at approximately 45 kDa, consistent with the predicted molecular weight of CPA1, with a secondary lower band that may represent proteolytic processing of this digestive enzyme, supporting its expected biochemical behavior in pancreatic tissue.