CAMK2A Antibody (R35142)

CAMK2A Antibody Human, Mouse and Rat Brain WB. Western blot testing of A) human cerebellum, B) mouse brain, and C) rat brain tissue lysates (35 ug protein per lane) with CAMK2A antibody. A distinct immunoreactive band is detected at approximately 54 kDa in all three brain lysates, consistent with the predicted molecular weight of Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2A). The results demonstrate cross-species detection of this neuronal serine/threonine kinase, which plays essential roles in calcium-dependent signaling, synaptic plasticity, learning, memory, and neuronal signal transduction.

CAMK2A Antibody Human Brain Cortex IHC. Immunohistochemical staining of FFPE human brain cortex tissue with CAMK2A antibody at 4 ug/ml using alkaline phosphatase (AP) detection. Heat-induced epitope retrieval (HIER) was performed by steaming tissue sections in pH 6 citrate buffer prior to staining. CAMK2A immunoreactivity is observed predominantly within cortical neurons and the surrounding neuropil, consistent with the expected distribution of Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2A), a neuronal serine/threonine kinase that regulates calcium-dependent signaling, synaptic plasticity, learning, and memory.

CAMK2A Antibody Human Adrenal Gland IHC. Immunohistochemical staining of FFPE human adrenal gland tissue with CAMK2A antibody at 3.75 ug/ml using alkaline phosphatase (AP) detection. Heat-induced epitope retrieval (HIER) was performed by steaming tissue sections in pH 6 citrate buffer prior to staining. CAMK2A immunoreactivity is observed predominantly within the cytoplasm of adrenal cortical cells, consistent with the expected expression of Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2A), a calcium signaling kinase that regulates calcium-dependent signal transduction, protein phosphorylation, and stimulus-responsive cellular signaling pathways.

CAMK2A Antibody U2OS Cell IF. Immunofluorescent staining of paraformaldehyde-fixed U2OS cells with CAMK2A antibody (green). Cells were permeabilized with 0.15% Triton X-100 prior to staining, followed by Alexa Fluor 488-conjugated secondary antibody. Nuclei are counterstained with DAPI (blue). CAMK2A immunoreactivity is observed predominantly within the cytoplasm, consistent with the expected localization of Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2A), a calcium signaling kinase that regulates calcium-dependent signal transduction, protein phosphorylation, and downstream cellular signaling pathways. The negative control using unimmunized goat IgG showed no specific staining.