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Home >> Antibodies >> Calnexin Antibody

Calnexin Antibody [clone CLNXN-1] (V3796)

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  Catalog No Formulation Size Price (USD)  
Image V3796-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3796-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3796SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC testing of FFPE human renal cell carcinoma with Calnexin antibody (clone CLNXN-1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human renal cell carcinoma with Calnexin antibody (clone CLNXN-1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Immunofluorescent staining of human HeLa cells with Calnexin antibody (green, clone CLNXN-1). RedDot used as nuclear counterstain (red).
Western blot testing of human HeLa cell lysate with Calnexin antibody (clone CLNXN-1). Predicted molecular weight ~68 kDa but routinely observed at ~90 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name CLNXN-1
Purity Protein G affinity chromatography
UniProt P27824
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Calnexin antibody is available for research use only.
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Description

Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse. [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the Calnexin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A partial human recombinant protein was used as the immunogen for the Calnexin antibody.

Storage

Store the Calnexin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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