BMP2 Antibody / Osteogenic Growth Factor Antibody (R31949)

BMP2 Antibody Multi-Species WB. Western blot analysis of 1) rat brain, 2) rat lung, 3) human U87, and 4) human HeLa lysates using BMP2 Antibody / Osteogenic Growth Factor Antibody demonstrates immunoreactive bands corresponding to multiple molecular forms of Bone Morphogenetic Protein 2 (BMP2). Bands are observed at approximately 22 kDa in rat brain and lung lysates and at approximately 44 kDa in human U87 and HeLa cell lysates. BMP2 is synthesized as a larger precursor protein that undergoes proteolytic processing and can exist in monomeric, dimeric, and precursor-associated forms, contributing to variability in apparent molecular weight. BMP2 functions as a member of the TGF-beta superfamily and plays critical roles in osteogenesis, skeletal development, tissue regeneration, and morphogenetic signaling. The observed immunoreactive species are consistent with the known post-translational processing and maturation of BMP2.

BMP2 Antibody Human Intestinal Cancer IHC. Immunohistochemistry staining of FFPE human intestinal cancer tissue using BMP2 Antibody / Osteogenic Growth Factor Antibody demonstrates moderate to strong cytoplasmic and membranous HRP-DAB brown staining within malignant epithelial cell populations forming glandular structures. The staining pattern is consistent with expression of Bone Morphogenetic Protein 2 (BMP2), a member of the TGF-beta superfamily that regulates cellular differentiation, tissue morphogenesis, growth factor signaling, and developmental pathways. Expression within tumor-associated epithelial cells is consistent with the established involvement of BMP signaling in epithelial biology, tissue remodeling, and regulation of cellular growth and differentiation. HIER: boil paraffin sections in pH 6, 10 mM citrate buffer for 20 minutes and allow to cool prior to staining.