• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> BMI1 Antibody

BMI1 Antibody [clone PGRF4-1] (V7401)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image V7401-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 559
Image
V7401-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 259
Image
V7401SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Image
V7401IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 559
Bulk quote request
›
Western blot testing of human 293T lysate with BMI1 antibody (clone PGRF4-1). Expected molecular weight: 37-43 kDa.
IHC testing of FFPE human colon carcinoma with BMI1 antibody (clone PGRF4-1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name PGRF4-1
Purity Protein G affinity chromatography
UniProt P35226
Localization Nuclear
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Prediluted IHC Only Format : incubate for 30 min at RT (1)
Limitations This BMI1 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

  • Applications : WB, IHC-P
    Reactivity : Human
    Microvalidated
  • Applications : IHC-P
    Reactivity : Human
    Microvalidated
  • Applications : WB, IF, IHC-P
    Reactivity : Human, Mouse
    Microvalidated
  • Applications : WB, IF, ELISA
    Reactivity : Human
  • Applications : WB
    Reactivity : Human
  • Applications : WB
    Reactivity : Human, Mouse, Rat
  • Applications : WB, IHC, ELISA
    Reactivity : Human
    Pred. Reactivity : Mouse, Bovine, Chicken
  • Applications : FACS, WB, IHC, IF, ELISA
    Reactivity : Human
  • Applications : WB, ELISA
    Reactivity : Human
  • Applications : WB, ELISA (peptide)
    Reactivity : Human
    Pred. Reactivity : Dog, Pig, Cow
  • Applications : WB, ELISA (peptide)
    Reactivity : Human, Mouse, Pig
    Pred. Reactivity : Dog

Description

The B cell-specific moloney murine leukemia virus integration site 1 (Bmi-1) is a transcriptional receptor of the polycomb gene family involved in several cellular processes including cell-cycle regulation, apoptosis, and maintenance of adult and neoplastic stem cells by providing self-renewal capacity. Further, Bmi-1 expression has been associated with malignant transformation, tumor progression, metastatic disease, and poor prognosis in human malignancies.

Additional studies involving polycomb-mediated transcriptional repression, stem cell renewal, and epigenetic regulation pathways may benefit from our BMI1 antibody page featuring clone BMI1/2823 with HuProt protein microarray specificity validation.

Application Notes

Optimal dilution of the BMI1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A portion of amino acids 142-326 from the human protein was used as the immunogen for the BMI1 antibody.

Storage

Store the BMI1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Related Products

   1.   View all BMI1 antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.