TUBB Antibody / Beta Tubulin Antibody (R32611)

TUBB Antibody Human, Zebrafish, Mouse and Rat WB. Western blot analysis was performed using TUBB Antibody on human HeLa and MCF-7 cell lysates (lanes 1-2), zebrafish embryo lysate (lane 3), rat brain tissue lysate (lane 4), and mouse brain and NIH3T3 cell lysates (lanes 5-6). Samples (30 ug) were separated by 10% SDS-PAGE under reducing conditions. The antibody was used at 0.125 ug/ml overnight at 4oC. A specific band was detected at approximately 50 kDa, consistent with the expected molecular weight of beta tubulin. This TUBB Antibody demonstrates detection of beta tubulin across human, zebrafish, mouse and rat samples.

TUBB Antibody Multi-Species Beta Tubulin WB. Western blot analysis was performed using TUBB Antibody on human SiHa, 293T and HepG2 cell lysates (lanes 1-3), monkey COS-7 cells (lane 4), chicken heart (lane 5), rat brain and PC-12 cells (lanes 6-7), and mouse brain and NIH/3T3 cells (lanes 8-9). Samples (30 ug) were separated by 5-20% SDS-PAGE under reducing conditions. The antibody was used at 0.5 ug/ml overnight at 4oC. A prominent band was detected at approximately 50 kDa, consistent with the expected molecular weight of beta tubulin. This TUBB Antibody demonstrates broad detection of beta tubulin across multiple vertebrate species and sample types.

TUBB Antibody Beta Tubulin Loading Control WB. Western blot analysis was performed using TUBB Antibody on human OCI-LY1 shCtrl and OCI-LY1 shSRPK1-2 whole cell lysates. Samples (30 ug) were separated by 5-20% SDS-PAGE under reducing conditions and transferred to a nitrocellulose membrane. The antibody was used at 1:3000 overnight at 4oC, followed by HRP-conjugated secondary antibody and ECL detection. A beta tubulin band was detected at approximately 55 kDa. Comparable detection across the samples demonstrates the use of this TUBB Antibody as a beta tubulin loading control in Western blot analysis.

TUBB Antibody Human Testicular Germ Cell Tumor IHC. IHC staining of FFPE human testicular germ cell tumor tissue was performed with TUBB Antibody at 2 ug/ml overnight at 4oC. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer, followed by HRP-conjugated secondary antibody and DAB detection. Positive beta tubulin staining is observed throughout the tumor tissue with a predominantly cytoplasmic pattern. This TUBB Antibody demonstrates localization of beta tubulin in human testicular germ cell tumor tissue.

TUBB Antibody Human Liver Cancer IHC. IHC staining of FFPE human liver cancer tissue was performed with TUBB Antibody at 2 ug/ml overnight at 4oC. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer, followed by HRP-conjugated secondary antibody and DAB detection. Positive beta tubulin staining is observed throughout the tumor cells with a predominantly cytoplasmic pattern. This TUBB Antibody demonstrates localization of beta tubulin in human liver cancer tissue.

TUBB Antibody Human Osteosarcoma Cell IF. Immunofluorescent staining of human U2OS cells was performed with TUBB Antibody at 5 ug/ml overnight at 4oC. Enzyme antigen retrieval was performed for 15 minutes, followed by Cy3-conjugated secondary antibody (red) and DAPI nuclear counterstain (blue). Strong beta tubulin staining highlights the cytoplasmic microtubule network throughout the cells. This TUBB Antibody demonstrates localization of beta tubulin and microtubule organization in human osteosarcoma cells.

TUBB Antibody Human Cervical Cancer Cell Flow Cytometry. Flow cytometry analysis of fixed and permeabilized human SiHa cells was performed with TUBB Antibody at 1 ug/million cells for 30 minutes at 20oC. The histogram shows cells alone (red), isotype control (green) and TUBB Antibody staining (blue). A clear fluorescence shift is observed with the TUBB Antibody compared with the isotype control. This TUBB Antibody demonstrates intracellular detection of beta tubulin in human cervical cancer cells.