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Home >> Antibodies >> Bcl-2 Antibody

Bcl-2 Antibody (F47422)

  Catalog No Formulation Size Price (USD)  
Image F47422-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 329
Image
F47422-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 135
Bcl-2 antibody western blot analysis in MDA-MB453 lysate
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Bovine, Chicken, Hamster, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P10415
Applications Western blot : 1:1000
Limitations This Bcl-2 antibody is available for research use only.
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Description

Bcl-2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). [UniProt]

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Bcl-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 144-171 from the human protein was used as the immunogen for this Bcl-2 antibody.

Storage

Can be stored at 4oC for one month. For long term, aliquot the Bcl-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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