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Home >> Antibodies >> ATG7 Antibody

ATG7 Antibody (F46229)

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  Catalog No Formulation Size Price (USD)  
Image F46229-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F46229-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Fluorescent image of Chloroquine treated U251 cells stained with ATG7 antibody at 1:200. Immunoreactivity is localized to autophagic vacuoles in the cytoplasm.
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Fluorescent image of Chloroquine treated U251 cells stained with ATG7 antibody at 1:200. Immunoreactivity is localized to autophagic vacuoles in the cytoplasm.
Western blot analysis of ATG7 antibody and 293 lysate transiently transfected with the human gene.
Western blot testing of ATG7 antibody and mouse liver tissue lysate. Predicted molecular weight: 70-80 kDa.
ATG7 antibody western blot analysis in HeLa lysate
IHC analysis of FFPE human hepatocarcinoma tissue stained with the ATG7 antibody
Availability 1-3 business days
Species Reactivity Human, Mouse
Predicted Reactivity Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt O95352
Applications Immunofluorescence : 1:200
Western blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Limitations This ATG7 antibody is available for research use only.
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Description

Autophagy is a process of bulk degradation of cytoplasmic components by the lysosomal/vacuolar system. ATG7 is a ubiquitin-activating enzyme E1-like protein essential for the Apg12 conjugation system that mediates membrane fusion in autophagy. [OMIM]

Application Notes

Titration of the ATG7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 494-523 from the human protein was used as the immunogen for this ATG7 antibody.

Storage

Aliquot the ATG7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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