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Home >> Antibodies >> ATG5 Antibody

ATG5 Antibody [clone 8F1-2C11-D9] (F54059)

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  Catalog No Formulation Size Price (USD)  
Image F54059-0.1ML In PBS with 50% glycerol and 0.03% ProClin 300 0.1 ml 399
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Western blot testing of 1) huma HCT116, 2) rat brain, 3) human K562 and 4) rat C6 cell lysates using ATG5 antibody at 1:1000. Predicted molecular weight ATG5: ~32 kDa; ATG5/ATG12 heterodimer: ~56 kDa.
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Western blot testing of 1) huma HCT116, 2) rat brain, 3) human K562 and 4) rat C6 cell lysates using ATG5 antibody at 1:1000. Predicted molecular weight ATG5: ~32 kDa; ATG5/ATG12 heterodimer: ~56 kDa.
Western blot testing of 1) rat brain and 2) human C2C12 cell lysates using ATG5 antibody at 1:1000. Predicted molecular weight ATG5: ~32 kDa; ATG5/ATG12 heterodimer: ~56 kDa.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse lgG1
Clone Name 8F1-2C11-D9
Purity Protein G affinity
UniProt Q9H1Y0
Applications Western blot : 1:1000
Limitations This ATG5 antibody is available for research use only.
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Description

Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. May play

Application Notes

The stated application concentrations are suggested starting points. Titration of the ATG5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A synthetic protein specific to ATG5 was used as the immunogen for the ATG5 antibody.

Storage

Store the ATG5 antibody at -20oC.

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