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Home >> Antibodies >> ATF1 Antibody

ATF1 Antibody (R31551)

  Catalog No Formulation Size Price (USD)  
Image R31551 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of ATF1 antibody and Lane 1: rat spleen; 2: human HeLa; 3: human COLO320. Routinely observed molecular weight: 29-35 kDa.
IHC-P: ATF1 antibody testing of human intestinal cancer tissue
Western blot testing of ATF1 antibody and recombinant human protein (0.5ng)
Availability 1-3 business days
Species Reactivity Human, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P18846
Gene ID 466
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This ATF1 antibody is available for research use only.
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Description

Activating transcription factor 1 is a protein that in humans is encoded by the ATF1 gene. This gene encodes an activating transcription factor which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. It is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation.

Application Notes

The stated application concentrations are suggested starting points. Titration of the ATF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 1-271) was used as the immunogen for this ATF1 antibody.

Storage

After reconstitution, the ATF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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