ATF1 Antibody / CREB Family Transcription Factor Antibody (R31551)

ATF1 Antibody U2OS Cell IF. Immunofluorescence analysis of ATF1 Antibody / CREB Family Transcription Factor Antibody in U2OS cells following enzyme antigen retrieval. ATF1 was detected with a Fluoro488-conjugated secondary antibody (green), while alpha tubulin was co-stained with a Cy3-conjugated secondary antibody (red) to visualize the cytoskeleton. ATF1 staining is predominantly nuclear, consistent with its function as a CREB family transcription factor. This ATF1 Antibody is suitable for investigating transcriptional regulation, signal transduction and protein localization.

ATF1 Antibody Human, Rat and Mouse WB. Western blot analysis of ATF1 Antibody / CREB Family Transcription Factor Antibody detected a major band at approximately 35 kDa, near the predicted 29 kDa molecular weight of ATF1. Lane 1: human HeLa; Lane 2: human A431; Lane 3: human SiHa; Lane 4: rat testis; Lane 5: mouse testis lysate. The membrane was incubated with ATF1 antibody at 0.5 ug/ml followed by an HRP-conjugated secondary antibody. This ATF1 Antibody detects endogenous ATF1 across human, rat and mouse samples and supports studies of CREB family transcriptional regulation.

ATF1 Antibody Human PC-3 FACS. Flow cytometric analysis of fixed and permeabilized human PC-3 cells stained with ATF1 Antibody / CREB Family Transcription Factor Antibody at 1 ug/million cells. Red: blank control; Green: isotype control; Blue: ATF1 antibody. The clear rightward shift of the ATF1 antibody population demonstrates specific detection of endogenous ATF1. This ATF1 Antibody is suitable for flow cytometric analysis of intracellular CREB family transcription factor expression in human cells.