Asc Antibody / Pycard / Tms1 (RQ8006)

Asc Antibody Mouse RM-1 Cell IF. Immunofluorescent staining of FFPE mouse RM-1 cells using Asc Antibody (red) and Alpha Tubulin monoclonal antibody (green). Asc is detected predominantly within the cytoplasm, consistent with its role as an inflammasome adaptor protein that mediates innate immune signaling, ASC speck formation, and inflammatory caspase activation. Alpha Tubulin staining highlights the microtubule cytoskeleton. Heat-induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 minutes prior to immunofluorescent staining.

Asc Antibody WB. Western blot analysis of 1) mouse lung, 2) mouse spleen, and 3) mouse thymus tissue lysates using Asc Antibody. A distinct immunoreactive band is detected at approximately 22 kDa across all three tissue lysates, consistent with the predicted molecular weight of Asc (PYCARD), supporting specific detection of the endogenous inflammasome adaptor protein in mouse tissues.

Asc Antibody RAW264.7 FACS. Flow cytometric analysis of fixed and permeabilized mouse RAW264.7 cells using Asc Antibody at 1 ug per million cells. A clear rightward shift of the antibody-stained population (blue) relative to the isotype control (green) demonstrates specific detection of endogenous Asc (PYCARD) expression. Cells alone are shown in red. Cells were blocked with goat serum prior to antibody incubation to minimize non-specific binding.