- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Related Products
|
Arginase-1 (ARG1), also referred to as arginase I or liver arginase, is a cytosolic enzyme encoded by the ARG1 gene that catalyzes the final step of the urea cycle, converting L-arginine into urea and ornithine. This metabolic reaction plays a central role in detoxification of ammonia and nitrogen metabolism in mammalian tissues. Arginase-1 is most abundantly expressed in hepatocytes of the liver, where the urea cycle functions to remove excess nitrogen generated during amino acid metabolism. Arginase 1 Antibody Protein Microarray Validated clone ARG1/1125 recognizes the ARG1 protein and supports research focused on arginine metabolism and liver-specific enzymatic pathways.
Arginase-1 belongs to the arginase enzyme family and functions as a manganese-dependent hydrolase localized primarily in the cytoplasm. The enzyme catalyzes hydrolysis of L-arginine to produce urea and L-ornithine, which subsequently contributes to polyamine and proline biosynthesis pathways. Because of this metabolic role, arginase-1 activity is tightly linked to hepatic nitrogen disposal and the maintenance of metabolic homeostasis.
ARG1 protein expression is strongly enriched in hepatocytes within normal liver tissue. The high level of hepatic expression makes arginase-1 a widely studied marker of hepatocellular differentiation in both normal liver biology and cancer research. Detection of arginase-1 protein is frequently used to investigate hepatocyte identity, liver metabolic function, and hepatocyte-derived tumor cells in experimental models.
Arginase-1 expression has also been investigated in the context of hepatocellular carcinoma and other liver-associated malignancies. Many hepatocyte-derived tumor cells retain ARG1 expression, making arginase-1 a useful biomarker when studying hepatocellular lineage and tumor cell differentiation. Detection of ARG1 protein allows researchers to examine metabolic enzyme expression in both normal and malignant liver cells while maintaining cellular context within tissue samples.
Clone ARG1/1125 has been validated using protein microarray analysis, a high-throughput approach used to evaluate antibody specificity across large panels of recombinant proteins. Protein microarray validation helps confirm that the antibody recognizes the intended ARG1 target with minimal cross-reactivity to unrelated proteins, supporting its use in research applications investigating arginase-1 expression.
Arginase 1 Antibody Protein Microarray Validated clone ARG1/1125 is a mouse monoclonal antibody designed to detect ARG1 protein in research applications. By enabling reliable detection of arginase-1, this antibody supports studies focused on hepatic metabolism, urea cycle biology, and cellular pathways involving arginine catabolism.
Optimal dilution of the Arginase 1 Antibody Protein Microarray Validated should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
A recombinant fragment from amino acids 1-150 of human ARG1 was used as the immunogen for the Arginase 1 antibody.
Store the Arginase 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Arginase-1 antibody, ARG1 antibody, Liver arginase antibody, Arginine ureahydrolase antibody
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.