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Home >> Antibodies >> Arginase 1 Antibody

Arginase 1 Antibody [clone ARG1/1125] (V2651)

  Catalog No Formulation Size Price (USD)  
Image V2651-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2651-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2651SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V2651IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
Western blot analysis of A) partial recombinant ARG1 protein and B) human liver lysate using Arginase 1 antibody (ARG1/1125). Predicted molecular weight ~35 kDa.
IHC: Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with Arginase 1 antibody (ARG1/1125). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT prior to testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Arginase 1 antibody (clone ARG1/1125). These results demonstrate the foremost specificity of the ARG1/1125 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free Arginase 1 antibody (clone ARG1/1125) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG3, kappa
Clone Name ARG1/1125
Purity Protein G affinity chromatography
UniProt P05089
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-4ug/ml for 30 min at RT
Limitations This Arginase 1 antibody is available for research use only.
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Description

Recognizes a protein of 35-38kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma.

Application Notes

Optimal dilution of the Arginase 1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A recombinant fragment from amino acids 1-150 of human ARG1 was used as the immunogen for the Arginase 1 antibody.

Storage

Store the Arginase 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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