AR Antibody / Androgen Nuclear Receptor Antibody (RQ5958)

AR Antibody in Human T-47D Breast Cancer Cells IF. Immunofluorescence testing of AR Antibody / Androgen Nuclear Receptor Antibody in FFPE human T-47D breast cancer cells. HIER was performed by steaming in pH 6 citrate buffer for 20 min. Strong androgen receptor immunoreactivity (green) is observed predominantly in the nuclei, with DAPI nuclear counterstaining shown in blue.

AR Antibody in Human A549 Lung Cancer Cells FACS. Flow cytometry testing of AR Antibody / Androgen Nuclear Receptor Antibody in human A549 lung cancer cells at 1 ug/million cells following blocking with goat sera. The AR antibody-stained population (blue) shows a strong fluorescence shift relative to the isotype control (green) and cells-alone control (red), demonstrating detection of androgen receptor under the tested conditions.

AR Antibody in Mouse RAW264.7 Macrophage Cells FACS. Flow cytometry testing of AR Antibody / Androgen Nuclear Receptor Antibody in mouse RAW264.7 macrophage cells at 1 ug/million cells following blocking with goat sera. The AR antibody-stained population (blue) shows a clear fluorescence shift relative to the isotype control (green) and cells-alone control (red), demonstrating detection of mouse androgen receptor under the tested conditions.

AR Antibody in Rat C6 Glioma Cells FACS. Flow cytometry testing of AR Antibody / Androgen Nuclear Receptor Antibody in rat C6 glioma cells at 1 ug/million cells following blocking with goat sera. The AR antibody-stained population (blue) shows a strong fluorescence shift relative to the isotype control (green) and cells-alone control (red), demonstrating detection of rat androgen receptor under the tested conditions.

AR Antibody in Human Cancer Cell Lysates WB. Western blot testing of AR Antibody / Androgen Nuclear Receptor Antibody in 1) K562, 2) U-2 OS, 3) HEK293, 4) PC-3, 5) 22Rv1, 6) HepG2 and 7) Caco-2 cell lysates. Androgen receptor is detected as a predominant band at approximately 100-120 kDa across the tested samples. The predicted molecular weight of AR is approximately 99 kDa, with migration above the calculated mass commonly observed for the full-length receptor.