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Home >> Antibodies >> APOBEC3A Antibody / PHO1

APOBEC3A Antibody / PHO1 (RQ4184)

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  Catalog No Formulation Size Price (USD)  
Image RQ4184 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of human 1) HeLa, 2) MCF7, 3) HepG2 and 4) mouse SP20 cell lysate with APOBEC3A antibody at 0.5ug/ml. Predicted molecular weight ~23 kDa, observed here at ~28 kDa.
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Western blot testing of human 1) HeLa, 2) MCF7, 3) HepG2 and 4) mouse SP20 cell lysate with APOBEC3A antibody at 0.5ug/ml. Predicted molecular weight ~23 kDa, observed here at ~28 kDa.
Flow cytometry testing of human A549 cells with APOBEC3A antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= APOBEC3A antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P31941
Applications Western Blot : 0.5-1ug/ml
Flow Cytometry : 1-3ug/10^6 cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This APOBEC3A antibody is available for research use only.
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Description

Apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A, also known as APOBEC3A, is a gene of the APOBEC3 family found in humans, non-human primates, and some other mammals.This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22.The protein plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. Two transcript variants encoding different isoforms have been found for this gene.

Application Notes

Optimal dilution of the APOBEC3A antibody should be determined by the researcher.

Immunogen

A recombinant human protein corresponding to amino acids M1-L63 was used as the immunogen for the APOBEC3A antibody.

Storage

After reconstitution, the APOBEC3A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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