APE1 Antibody CPTC-APEX1-2 / AP Endonuclease 1 Antibody [clone CPTC-APEX1-2] (V7287)

APE1 Antibody CPTC-APEX1-2 Detection in Human Cancer Cell Lines WB. Western blot testing of human A431, A549, PC-3, HAP1 and HepG2 cell lysates with APE1 Antibody clone CPTC-APEX1-2 shows a strong, discrete band at approximately 35-37 kDa across all five samples, consistent with the predicted molecular weight of ~38 kDa for AP endonuclease 1. The clean, target-sized signal across multiple cell types supports detection of endogenous APE1.

APE1 Antibody CPTC-APEX1-2 Staining of HeLa Cervical Cancer Cells IF. Immunofluorescence staining of permeabilized human HeLa cervical cancer cells with APE1 Antibody clone CPTC-APEX1-2 shows strong green APE1 signal concentrated in the nuclei, with additional cytoplasmic staining. Red phalloidin staining delineates the actin cytoskeleton and cellular architecture.

APE1 Antibody CPTC-APEX1-2 Staining of Human Prostate Carcinoma IHC. Immunohistochemistry testing of FFPE human prostate carcinoma with APE1 Antibody clone CPTC-APEX1-2 shows prominent brown nuclear staining throughout the carcinoma cells, with variable additional cytoplasmic immunoreactivity. HIER was performed by steaming sections in pH 6 citrate buffer for 20 min followed by cooling before antibody testing.

AP Endonuclease 1 Antibody CPTC-APEX1-2 Staining of Human Prostate Carcinoma IHC. Immunohistochemistry testing of FFPE human prostate carcinoma with AP Endonuclease 1 Antibody clone CPTC-APEX1-2 shows strong brown staining of the glandular epithelium, including prominent nuclear and cytoplasmic immunoreactivity, with substantially less staining in the surrounding stromal tissue. HIER was performed by steaming sections in pH 6 citrate buffer for 20 min followed by cooling before antibody testing.

APE1 Antibody CPTC-APEX1-2 Detection in MCF7 Breast Cancer Cells FACS. Flow cytometry testing of human MCF7 breast cancer cells with APE1 Antibody clone CPTC-APEX1-2 shows a pronounced rightward fluorescence shift of the antibody-stained population (blue) relative to the isotype control (red), demonstrating intracellular detection of APE1.

APE1 Antibody CPTC-APEX1-2 Protein Microarray Validation. Protein microarray analysis of APE1 Antibody clone CPTC-APEX1-2 using a HuProt array containing more than 19,000 full-length human proteins identifies APEX1 as the dominant binding target. APEX1 ranks first with a Z-score of 100.7 and S-score of 71.49, showing strong separation from the next-highest protein signal. The Z-score represents target-binding signal relative to the array mean, while the S-score measures separation from the next-highest target, supporting the specificity of this Protein Microarray Validated antibody.

APE1 Antibody CPTC-APEX1-2 Purity Analysis by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free APE1 Antibody clone CPTC-APEX1-2 with 2 ug loaded under reduced (R) and non-reduced (NR) conditions. Reduction produces the expected immunoglobulin heavy and light chains at approximately 50 kDa and 25 kDa, while the non-reduced antibody migrates predominantly near 150 kDa, consistent with intact IgG. The defined banding pattern supports the integrity and purity of the monoclonal antibody preparation.